Chemistry & Chemical Reactivity
Chemistry & Chemical Reactivity
10th Edition
ISBN: 9781337399074
Author: John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher: Cengage Learning
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Chapter 24.5, Problem 2.3ACP

(a)

Interpretation Introduction

Interpretation:

The primers frequently chosen that have high number of cytosines and guanines in their sequences; the reason has to be predicted.

Concept introduction: Cytosine is a nucleotide found in DNA and RNA. It is a derivative of pyrimidine with heterocyclic aromatic ring. Guanine is the one of the four bases in DNA and RNA. It is dervative of purine. Guanine always pairs with cytosine. Guanine and Cytosine makes three hydrogen bonds.

Primers are short strand(18-20 base pairs) of DNA and RNA which helps in initition of DNA synthesis. The main role of primer is to allow polymerase to start because it cannot start by itself.

(b)

Interpretation Introduction

Interpretation:

The primer sequence of CCCCCCCCCCGGGGGGGGGG not is a good choice; the reason has to be predicted.

Concept introduction: Cytosine is a nucleotide found in DNA and RNA. It is a derivative of pyrimidine with heterocyclic aromatic ring. Guanine is the one of the four bases in DNA and RNA. It is dervative of purine. Guanine always pairs with cytosine. Guanine and Cytosine makes three hydrogen bonds.

Primers are short strand(18-20 base pairs) of DNA and RNA which helps in initition of DNA synthesis. The main role of primer is to allow polymerase to start because it cannot start by itself.

(c)

Interpretation Introduction

Interpretation:

Vast excess of primer used for the amount of DNA has to be amplified.

Concept introduction: Cytosine is a nucleotide found in DNA and RNA. It is a derivative of pyrimidine with heterocyclic aromatic ring. Guanine is the one of the four bases in DNA and RNA. It is dervative of purine. Guanine always pairs with cytosine. Guanine and Cytosine makes three hydrogen bonds.

Primers are short strand(18-20 base pairs) of DNA and RNA which helps in initition of DNA synthesis. The main role of primer is to allow polymerase to start because it cannot start by itself.

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Are the following compounds correct? These may OR may not be correct. Please look them over and correct them if needed. Please list what was done incorrectly if applicable. b. Is the following nucleotide found in DNA? If not, please explain why. a. Is the following tRNA correct? NH2 VALINE <5' N. 3'- 0=P-0-CH2 ОН ОН GUA a. b. DNAA=T G=C
Important steps in sequencing pure proteins include all of these except: a. Determining the amino acids on the ends of the smaller peptides b. Determining the amino acids on the ends of the protein c. Determining the isoionic pH of the protein d. Determining the amino acid composition e. Breaking the protein into smaller peptides   It is impossible to sequence a protein if you do not have overlapping sequences to work with. A. True B. False
What is NOT a feature of PCR? O a. The target can be much larger than the primers. Ob. Primers do not have to be perfectly matched to flanking sequences to amplify targets. O. PCR is highly specific because of the stringency of hybridization at relatively high temperature. d. The sequence of the target needs to be known so that complementary primers can be synthesized. Oe. PCR is exquisitely sensitive; a single DNA molecule can be amplified and detected.

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Chemistry & Chemical Reactivity

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