Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781259709227
Author: Marjorie Kelly Cowan Professor, Heidi Smith
Publisher: McGraw-Hill Education
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 2, Problem 1VC
Summary Introduction
To explain:
Whether isolated colonies will be visible in quadrant 4 and quadrant 3 in the given illustration while using quadrant streak plate method to plate a very dilute broth culture.
Introduction:
Streaking is a microbiological technique which is used for isolation of pure strain from single species of microorganism. It is an important method used for isolation and determination of bacteria in clinical sample.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Why is it important to use a sterilized loop between streaks when preparing a streakplate?
Observation of a streakplate culture shows more growth in Quadrant 4 than in Quadrant 3. Account for this observation.
Describe the way in which you can isolate an individual colony from a spreadplate or a streakplate that holds multiple colonies.
Outline the differences between a streak plate and a spread plate.
If you were using the quadrant streak plate method to plate a very dilute broth culture ( with many fewer bacteria the broth used for the plate picture here), would you expect to see single, isolated colored in quadrant 4 or quadrant3? Explain your answer.
1) based on the difference in appearance between mixed culture streak plates and broth culture plates, what would lead you to believe that you have created a pure culture when you inoculated the broths?
2) when using the loop dilution technique to produce pour plates, did all the plates produce isolated colonies? If your goal was to ultimately create pure cultures of E. coli, M. luteus, and S. morcescens, which plate or which dilution would you use, and why would you use this plate?
3) What are the advantages and disadvantages of streak plating compared with pour plating?
Chapter 2 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 2.1 - Explain what the Five Is are and what each step...Ch. 2.1 - Discuss three physical states of media and when...Ch. 2.1 - Compare and contrast selective and differential...Ch. 2.1 - Provide brief definitions for defined media and...Ch. 2.1 - Medical Moment The Making of the Flu Vaccine: An...Ch. 2.1 - Prob. 1NPCh. 2.1 - Prob. 2NPCh. 2.2 - Prob. 5AYPCh. 2.2 - Prob. 6AYPCh. 2.2 - Prob. 7AYP
Ch. 2.2 - Give examples of simple, differential, and special...Ch. 2.2 - Prob. 3NPCh. 2.2 - Medical Moment Gram-Positive Versus Gram-Negative...Ch. 2 - The identities of microorganisms on our planet a....Ch. 2 - Prob. 2QCh. 2 - Often bacteria that are freshly isolated from a...Ch. 2 - Which of these types of organisms is least likely...Ch. 2 - Prob. 5QCh. 2 - Some bacteria can produce a structure called an...Ch. 2 - A fastidious organism must be grown on what type...Ch. 2 - Write a short paragraph to differentiate among the...Ch. 2 - Prob. 9QCh. 2 - Viruses are commonly grown in/on a. animal cells...Ch. 2 - Can you devise a growth medium with ingredients...Ch. 2 - There is a type of differential medium that can...Ch. 2 - Prob. 13QCh. 2 - Several bacteria live naturally in a material on...Ch. 2 - Archaea often grow naturally in extreme...Ch. 2 - Prob. 16QCh. 2 - After performing the streak plate procedure on a...Ch. 2 - You are a scientist studying a marsh area...Ch. 2 - Prob. 19QCh. 2 - Prob. 20QCh. 2 - You perform the special stain for bacterial...Ch. 2 - Prob. 1VC
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply) a - There is fungal growth in the original broth culture tube. b- There is too much growth on the agar slant. c- There are colonies of similar morphology on the slant. d - There are red, yellow, and white colonies on the slant. e - There is no growth on the slant.arrow_forwardA soil sample is placed in liquid and the number of bacteria in the sample determined in two ways: (1) colony count and (2) direct microscopic count. How would the results compare?a) Methods 1 and 2 would give approximately the same results.b) Many more bacteria would be estimated by method 1.c) Many more bacteria would be estimated by method 2.d) Depending on the soil sample, sometimes method 1 would be higher and sometimes method 2 would be higher.arrow_forwardYou are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?arrow_forward
- There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one has been incubated for 24 hours. After a 10x dilution by taking 100µl of each culture and adding it to 900 µl water in a microcentrifuge tube and 100µl sample from the tube was taken to view in the counting chamber. a) Count the total number of yeast cells for each culture respectively b) Calculate the concentration and density of yeast cells for each culture respectivelyarrow_forwardHow does the streak plate technique help in isolating individual colonies of bacteria? Why streak multiple times on one plate? 3. While observing a plate inoculated with bacteria you observe a white-tan fuzzy growth. What can you interpret from this observation? Explain how this outcome could’ve occurred. 4. What are 3 possible errors that could take place as you culture your samples which could lead to contamination? State 3 and explain why. Microbiology classarrow_forwardIn a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with moldarrow_forward
- You were asked to prepare a dilution series of a bacterial culture where only 3 tubes will be used (all with 5 mL total solution). Draw a schematic diagram to make tube 1 have a 10-2 dilution, tube 2 have 10-3 dilution, and tube 3 to have 10-5 dilution You were instructed to dilute an antibiotic solution 1/10, redilute 1/25, and again 1/50, then you need to make 100mL of each dilution. How would you go about preparing this dilution series? (Present your answer in a schematic diagram)arrow_forwardUsing a dilution series is a way to transfer the very small volumes of original culture necessary to get countable colony forming units. If you suspect your culture of bacteria has 59.62*10^9 cells per mL, how many mL of original culture would you want to end up on the plate to produce 68 cfu? Express your answer in scientific notation rounded to two decimal places. Eg. 0.01001 would be 1.00*10^-2 You must include trailing zeros 0.0000040001 would be reported as 4.00*10^-6 There must always be two decimal places, even if they are zeros Canvas expects one digit before the decimal point 0.40*10^-5 would be marked incorrect for 0.0000040001arrow_forwardGiven the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________arrow_forward
- What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?arrow_forwardYou are tasked with quantifying bacteria in your soil sample. You resuspend 1.0g of soil in 10 ml 1x PBS, then do a 10-fold serial dilution of your soil sample. You plate 150 µl onto each plate. The 104 dilution plate contains 175 colonies after 2 days of incubation, how many bacteria do you estimate were present in your initial soil sample? (# colonies/volume plated in ul) * dilution factor * ul convert to ml * volume suspended soil (ml/g) = Read the formatting instructions below before entering your answer! 1. Make sure your answer is in correct scientific notation using an "e" to indicate the exponent! For example, 2500 would be 2.5e3, 0.0066 would be 6.6e-3. 2. For answers greater than 1, round up to the nearest tenth. For example, 2515 would be 2.5e3 and 51510 would be 5.2e4.arrow_forwardOn agar plate does each discrete colony represent the growth of one cell? Explain your answer. Why can a single colony on a plate be used to start a pure culture?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education