Q: What advantages do solid media offer for the isolation ofmicroorganisms?
A: Micro-organism are those that cannot be seen by the naked eye, but are visible under microscopes.…
Q: What is a mixed culture? A contaminated culture?
A: A microbiological culture, or microbial culture, is a technique for increasing microbial creatures…
Q: What is the purpose of flame-sterilizing the mouth of the test tube and the side of the Petri dish…
A: Flame is an excellent way of cleaning a surface from contaminating organisms. Instant high heat…
Q: Why don't you get isolated colonies from the pour plate if the broth culture was not diluted first?
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: Why is the level of contamination measured as number of colonies rather than size of colonies?
A: Microbiological contamination refers to the presence of unwanted or accidental introduction of…
Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…
A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…
Q: Why does a streaking method used to inoculate plates result in isolated colonies?
A: Streaking method is used to obtain pure colonies of microbes(bacteria) from a proper single species…
Q: What is the difference between culture-based technique and culture-independent techniques based on…
A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…
Q: Are the large numbers of microorganisms found in the mouth cause for concern? Explain.
A: We are authorized to answer one question at a time.Since you have not mentioned which one,I am…
Q: Why is the culture medium referred to as a highly enriched laboratory soil?
A: Culture medium is referred to as highly enriched laboratory soli because it provides all the…
Q: Why is it important for a microbiology laboratory technician to use aseptic technique?
A: The majority of the microbes onto which scientists work in laboratories are known pathogens.
Q: Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?
A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…
Q: What is the difference between the pour plate method and the spread plate method? Why might you…
A: Standard plate count method is a growth based approach that counts the number of living…
Q: How can the enrichment culture technique be used medically?
A: Bacteria require appropriate environmental conditions for its growth. Solid or a liquid preparation…
Q: Why are semi-solid media such as TTC deeps suitable to test motility of bacteria?
A: There are several parameters which need to be studied in microbiology about different…
Q: What is culture media? Which culture media is used for gram negative bacteria and which one for gram…
A: Microbiology is the branch of science which deals with the study of different types microorganisms…
Q: Why are complex media preferable to chemically defined media for routinecultivation of…
A: Microorganisms require nutrients as an energy source and certain other environmental conditions to…
Q: What is the difference between streak plate method and spread plate technique?
A: Answer of the question given below...
Q: CTSA growth medium designed
A: Under the regulated conditions of the laboratory when microbial cells are allowed to grow and…
Q: If SIM medium was used for motility determination for Proteus vulgaris, what noticeable change to…
A: What is motility? Motility is the ability of an organism to move by itself. Eucaryotic cells use…
Q: For the pour plate method, state which plate will be chosen for colony isolation and why? From the…
A: Streak plate method and pour plate method is used to isolate bacteria
Q: Why should culture media after inoculation be incubated at an optimal temperature immediately?
A: Culture media, also called as growth media, are particular combinations of nutrients and other…
Q: temperature and light source affect the number and type of colonies that can be found in the…
A: Microbes such as bacteria, fungi, viruses are grown at the earth's surface. The atmosphere provides…
Q: Why is it important to follow sterile technique?
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: what is the culture media for gram-positive bacteria?
A: The culture media for gram positive bacteria is Mannitol salt agar . The presence of bile salts, as…
Q: Why did we both stab and streak our blood agar plates when inoculating them with bacteria?
A: Blood agar is a nutrient medium which contain essential nutrients for bacterial growth and blood of…
Q: What is the basis of enrichment culture techniques? Why is an enrichment medium usually suitable for…
A: Enrichment culture media is the one which is used for the growth and isolation of a specific type of…
Q: What is a pure culture and how can one be obtained
A: Cells are the structural and functional units of life.
Q: How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial…
A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…
Q: How would you expect the staining properties of 24-hour culture of Bacillus subtilis or the other…
A: Bacillus subtilis is a very well bacteria that is widely used as a Gram-positive bacteria model…
Q: What is the name of this media? What type of media is it? What does the yellow color indicate? What…
A: Selective media generally selects for the growth of a desired organism, stopping the growth of or…
Q: What is the advantage of the plating method over an electronic cell counting method in counting…
A: Bacteria culturing is done in order to grow the bacterial strains that are able to show resistance…
Q: What is the main advantage of using the slide culture technique for identifying molds?
A: Side Culture - It is a rapid method of preparing fungal colonies for examination and identification.…
Q: Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?
A: Gram staining is a differential staining technique developed by Danish bateriologist Hans Christian…
Q: What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic…
A: Endospore stain is a differential stain that is used to image endospores in bacteria. A few genera…
Q: Why does dry heat require higher temperatures for longer time periods to sterilize than moist heat
A: Sterilization may be defined as the process which help in the complete removal of all…
Q: How does the agar dilution method differ from streaking toobtain isolated colonies?
A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…
Q: What are the advantages and disadvantages of using the Slide Culture technique?
A: Slide culture technique is mainly utilized for the preparation of fugal colonies. Fungi are…
Q: How do we isolate culture media (step by step process) and what is the purpose of bacterial culture…
A: Introduction :- Microorganisms can be found in the natural environment, such as soil. They coexist…
Q: the chemicals used in the gram staining method?
A: The bacteriologist Hans Christian Gram was developed gram staining method in 1884. the Gram staining…
Q: What microbe requires serum components to be added to the growth medium?
A: Introduction Microorganisms:- An organism that can be seen only through a microscope, They live in…
Q: You have a starter culture containing 8 x 109 cells per mL, from which you take 10 mL to inoculate a…
A: The generation time of culture is considered to be the time at which it doubles and the mean growth…
Q: Why does a streaking method used to inoculate plates result in isolated colonies? (Explain…
A:
Q: Can pure culture be prepared directly from a mixed culture? Explain your answer.
A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…
Q: If you were using the quadrant streak plate method to plate a very dilute broth culture (with many…
A: Streak plate technique is the technique or method that is widely used for the isolation of a pure…
Q: What method can be used to achieve sterilization?
A: It is required to describe the method to be used to achieve sterilization.
Q: explain why you would expect to see similarities in the types of colonies on the fomite plates and…
A: Organisms that are too small to be seen by the naked eye are microorganisms. These organisms can be…
Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?
A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…
What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?
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- Why does a streaking method used to inoculate plates result in isolated colonies? (Explain systematically)In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?Why does a streaking method used to inoculate plates result in isolated colonies?
- Why go to the trouble of creating a master plate (why not simply plate the initial culture on both nutrient agar and glucose-salts agar)?In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?Why is dilution a necessary part of pure culture preparation?
- during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.Why do you think the continuous culture has higher chances of contamination than batch and fed-batch culture?what is the physical characteristics of this streak plate? Look at a single colony on a streak plate and look for special physical characteristics such as: motility, possible presence of endospores and/or capsule, culture color?
- Why is it important to keep the culture at the correct incubation temperature when measuring the generation time?You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?What is a mixed culture? A contaminated culture?