Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781259709227
Author: Marjorie Kelly Cowan Professor, Heidi Smith
Publisher: McGraw-Hill Education
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Chapter 2, Problem 5Q
Summary Introduction
To determine:
Whether the functions of microorganisms can be revealed with the help of microscopy techniques
Concept introduction:
Microscopy is the technique which is used to identify cells and microbes under the microscopes. Various structures and functions can be observed with the help of microscopes.
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Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope.
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Chapter 2 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 2.1 - Explain what the Five Is are and what each step...Ch. 2.1 - Discuss three physical states of media and when...Ch. 2.1 - Compare and contrast selective and differential...Ch. 2.1 - Provide brief definitions for defined media and...Ch. 2.1 - Medical Moment The Making of the Flu Vaccine: An...Ch. 2.1 - Prob. 1NPCh. 2.1 - Prob. 2NPCh. 2.2 - Prob. 5AYPCh. 2.2 - Prob. 6AYPCh. 2.2 - Prob. 7AYP
Ch. 2.2 - Give examples of simple, differential, and special...Ch. 2.2 - Prob. 3NPCh. 2.2 - Medical Moment Gram-Positive Versus Gram-Negative...Ch. 2 - The identities of microorganisms on our planet a....Ch. 2 - Prob. 2QCh. 2 - Often bacteria that are freshly isolated from a...Ch. 2 - Which of these types of organisms is least likely...Ch. 2 - Prob. 5QCh. 2 - Some bacteria can produce a structure called an...Ch. 2 - A fastidious organism must be grown on what type...Ch. 2 - Write a short paragraph to differentiate among the...Ch. 2 - Prob. 9QCh. 2 - Viruses are commonly grown in/on a. animal cells...Ch. 2 - Can you devise a growth medium with ingredients...Ch. 2 - There is a type of differential medium that can...Ch. 2 - Prob. 13QCh. 2 - Several bacteria live naturally in a material on...Ch. 2 - Archaea often grow naturally in extreme...Ch. 2 - Prob. 16QCh. 2 - After performing the streak plate procedure on a...Ch. 2 - You are a scientist studying a marsh area...Ch. 2 - Prob. 19QCh. 2 - Prob. 20QCh. 2 - You perform the special stain for bacterial...Ch. 2 - Prob. 1VC
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- a. How was the specimen prepared for the microscopy technique applied? (for e.g. stained with H&E stain, Gram stain, unstained) b. What is the microscopy technique and magnification used to obtain this image? c. What is the basic principle of image formation using this microscopy technique? d. What can be observed and concluded from the image of the specimen? e. Are there any potential aberrations present in this specimen image? Describe these and how they may affect interpretation of the result.arrow_forwardIf you were trying to visualize living organisms without staining, which type of microscopy would be best to utilize? O 1) brightfield O 2) TEM 3) fluorescent O 4) confocal O 5) phase contrastarrow_forwardWhat limitation applies to most forms of electron microscopy?arrow_forward
- How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensityarrow_forwarda) Briefly describe the concept of and instrument configuration for confocal microscopy. b) How do confocal and conventional microscopy compare? c) What other microscopy techniques can provide super-resolution?arrow_forwardFor which types of specimens is darkfield microscopy preferred over brightfield microscopy?arrow_forward
- Discuss the microscopy technique that gives the best contrast image of non-biological samples such as micro and nano-particles.arrow_forwardCompare the appearance of microorganisms you observed using phase-contrast microscopy versus brightfield microscopy.arrow_forwardWhat are the differences between phase contrast microscopy and differential interference microscopy?arrow_forward
- In light microscopy, when magnification is increased, the lens focuses closer to the sample. This makes the user more likely to ram the sample into the lens. Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?arrow_forwardRead the following scenarios carefully and determine which type of microscope is the most applicable to use. A scientist is planning to observe a type of herpes virus under a microscope. He wants to assess if the specimen he is currently working on is the Epstein-Barr Virus, which is a type of Herpes Virus. What do you think is the most useful type of microscope for the scientist to use?arrow_forwardWhat is phase contrast? Give examples of phase contrast in optical microscopy.arrow_forward
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