Developmental Biology
11th Edition
ISBN: 9781605354705
Author: Scott F. Gilbert, Michael J. F. Barresi
Publisher: Sinauer Associates is an imprint of Oxford University Press
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Chapter 5, Problem 7DQ
Summary Introduction
To review:
The possibility that naïve human ESCs can be isolated and maintained. The ethical concerns could such studies raise.
Introduction:
The developmental stages of the original inner cell mass (ICM) from which the cultures were derived has led to recognition of two different pluripotent states of an ESC: naïve and primed. The naive Embryonic Stem Cell (ESC) represents the most immature, undifferentiated ESC with the potential for pluripotency. The primed Embryonic Stem Cell (ESC) represents an ICM cell with some maturation during post-implantation development.
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Task #1 Mc1r alleles: In Florida Gulf coast mice, there are two different alleles of the
Melanocortin Receptor (Mcr1) gene. The sequence for both alleles is shown below. This
is an internal segment of the coding (non-template) sequence of the Mcr1 gene. The
reading frame is set and you do not need to find an AUG.
M allele
m allele
5'...ATC ACC AAA AAC CGC AAC CTG CAC TCG...
5'...ATC ACC AAA AAC TGC AAC CTG CAC TCG...
A. Compare these two allele sequences and circle the nucleotides that are different.
B. Do you think this change will cause
mRNA Codon Table
Second base
a shift in the codon reading frame?
Why or why not?
C. Do you think that this change will
cause an early stop in translation?
Why or why not?
First base
U
U Phe
Phe
Leu
Leu
C Leu
Leu
Leu
Leu
A lle
lle
lle
Met
G Val
Val
Val
Val
C
Ser
Ser
Ser
Ser
Pro
A
G
Cys
Tyr Cys
STOP Trp
STOP STOP A
His Arg
Pro
His
Pro Gin
Pro Gin
Thr
Asn
Thr
Asn
Thr
Lys
Thr
Lys
Ala
Ala
Ala
Ala
Arg
Arg
Arg
Ser
Ser
Arg
Arg
Gly
Asp
Asp
Glu Gly
Glu
Gly…
Lesson 2
Focus Questions
1. What chemicals and molecules are needed for PCR, and what is the function of each
component?
2. Examine the 150 base promoter sequence below.
Kaylee Kauff
5'TAGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA TAAAGGAAAG
GTATCATTC AAGATGCCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC
ACCCACGAGG AGC ATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA3'
Write in the sequence of the complementary strand and mark the 3' and 5' ends of the
complementary strand.
43
Question:
What is a loss of function of a certain protein that prevents nucleotide metabolism and prevents DNA synthesis - this gene is expressed in all tissues but is expressed at the highest levels in bone marrow?
Chapter 5 Solutions
Developmental Biology
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