Biology 2e
2nd Edition
ISBN: 9781947172517
Author: Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher: OpenStax
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Textbook Question
Chapter 17, Problem 14RQ
The chain termination method of sequencing
- uses labeled ddNITPs
- uses only dideoxynucleotides
- uses only deoxynucleotides
- uses labeled dNTPs
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Students have asked these similar questions
A Sanger product of the sequencing of a template DNA is presented
+ddTTP
+ddATP
+ddCTP
+ddGTP
Mononucleotide Pentanucleotide Trinucleotide
Dinucleotide
11-nucleotide
Hexanucleotide Octanucleotide Tetranucleotide
16-nucleotide
Decanucleotide Nonanucleotide Heptanucleotide
17-nucleotide
15-nucleotide
13-nucleotide
12-nucleotide
18-nucleotide
20-nucleotide
14-nucleotide
19-nucleotide
21-nucleotide
1. Determine the sequence of template DNA
2. Determine the mRNA sequence from this template DNA
3. Determine the the sequence of protein product derived from that DNA
What is the DNA template of the following DNA coding:
ATGGCTAACCTTGTA
Sequencing reactions are done in separate tubes for each ddNTP with a radioactive primer. Which picture shows the correct 4 reactions after separation of the sequencing reaction by gel
electrophoresis?
Template:
Primer:
5'-ATCGCTTACCATTAG-3'
5'-CTAAT-3'
ddA ddC ddG ddT
ddA
ddC ddG ddT
=
D
ddA ddC ddG ddT
A
ddA ddC ddG ddT
—
B
C
Chapter 17 Solutions
Biology 2e
Ch. 17 - Figure 17.7 You are working in a molecular biology...Ch. 17 - Figure 17.8 Do you think Dolly was a Finn-Dorset...Ch. 17 - Figure 17.15 In 2011, the United States...Ch. 17 - GMOs are created by. generating genomic DNA...Ch. 17 - Gene therapy can be used to introduce foreign DNA...Ch. 17 - Insulin produced by molecular cloning is of pig...Ch. 17 - Bt toxin is considered to be. a gene for modifying...Ch. 17 - The Flavr Savr Tomato: is a variety of...Ch. 17 - generated after a cDNA library is made unique...Ch. 17 - Linkage analysis. is used to create a physical map...
Ch. 17 - Genetic recombination occurs by which process?...Ch. 17 - Individual genetic maps in a given species are:...Ch. 17 - Information obtained by microscopic analysis of...Ch. 17 - The chain termination method of sequencing uses...Ch. 17 - Whole-genome sequencing can be used for advances...Ch. 17 - Sequencing an individual person’s genome is...Ch. 17 - What is the most challenging issue facing genome...Ch. 17 - Genomics can be used in agriculture to generate...Ch. 17 - Genomics cam be used on a personal level to:...Ch. 17 - What is a biomarker? the color coding of different...Ch. 17 - A protein signature is: the path followed by a...Ch. 17 - Describe the precess of Southern blottingCh. 17 - A researcher wants to study cancer cells from a...Ch. 17 - How would a scientist introduce a gene for...Ch. 17 - If you had a chance to get your genome sequenced,...Ch. 17 - Why is so much effort being poured into genome...Ch. 17 - How could a genetic map of the human genome help...Ch. 17 - Explain why metagenomics is probably the most...Ch. 17 - How can genomics be used to predict disease risk...Ch. 17 - How has proteomics been used in cancer detection...Ch. 17 - What is personalized medicine?
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- Use the gel image below to determine the sequence of the original DNA template strand sequenced using Sanger sequencing from the 5' end to the 3' end. C G T TAC GGT CAT TAC TGG CAT ATG CCA GTA ATG ACC GTA TAC GGT TACarrow_forwardOrder the steps required to sequence a region of DNA using dideoxy sequencing. Amplify the region of DNA to be sequenced add a primer, deoxynucleotides, labeled dideoxynucleotides, and DNA polymerase a primer binds to the single-stranded DNA template DNA polymerase extends the primer, incorporating deoxynucleotides a labeled dideoxynucleotide terminates the growing DNA chain gel electrophoresis separates the mixture of DNA fragments by size The DNA sequence is determined denature the double-stranded DNA Answer Bankarrow_forwardDraw the gel electrophoretic pattern that would be seen in dideoxy sequenceanalysis of the DNA moleculearrow_forward
- What are plasmids? What are sticky ends? What is the function of Ligase? DNA Gel electrophoresis is conducted on what principles? Write at least 3.arrow_forwardWhich of the following functions are associated with the E. coli RNA polymerase? Select all that apply Helicase activity Topoisomerase activity 3' to 5' polymerase activity 5' to 3' polymerase activityarrow_forwardPlease fill out the following table. Circle sense or template strands. Indicate 5’, 3’, amino and carboxy ends. Use wobble whenever possiblearrow_forward
- Choose the right combination of components required to set up a polymerase chain reaction from the following: Template RNA, two primers, NTPs, and DNA polymerase Template DNA, two primers, dNTPs, and DNA ligase Template DNA, two primers, NTPs, and DNA ligase Template DNA, two primers, dNTPs, and DNA polymerasearrow_forwardPlace the steps of base excision repair in order. Not all choices will be used. hydrolyze base First step recognize incorrect base separate strands using helicase hydrolyze incorrect nucleotide using endonuclease fill in gap with DNA polymerase close DNA ends with DNA ligase Last step Answer Bank hydrolyze abasic nucleotide using endonucleasearrow_forwardWhich of the following sequences, in the double-stranded form, can probably be recognized by a restriction enzyme? Choose an answer from below? TTCCTT CGACGA GTCGAC GGTTGG GAGGACarrow_forward
- Sequencing reactions are done in separate tubes for each ddNTP with a radioactive primer. Which picture shows the correct 4 reactions after separation of the sequencing reaction by gel electrophoresis? (Hint: Primer sequence cannot be read from the gel.) Template: Primer: 5'-ATCGCTTACCATTAG-3' 5'-CTAAT-3' ddA ddC ddG ddT ddA ddC ddG ddT = — ddA ddC ddG ddT = A B O None of these figures A ddA ddC ddG ddT B C Darrow_forwardEnzymes of bacterial origin used in a wide variety of techniques are: ligases restriction endonucleases primase dna polymerase resctriction exonucleasearrow_forwardWhich of the following is correct regarding sequencing by synthesis? All four nucleotides are added simultaneously during the sequencing phase. All four nucleotides have fluorescent tags. Homopolymer error rates are significantly lower than other methods of second-generation sequencing. All of the abovearrow_forward
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