Biology 2e
2nd Edition
ISBN: 9781947172517
Author: Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher: OpenStax
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Textbook Question
Chapter 17, Problem 13RQ
Information obtained by microscopic analysis of stained chromosomes is used in:
- radiation hybrid mapping
- sequence mapping
- RFLP mapping
- cytogenetic mapping
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Chapter 17 Solutions
Biology 2e
Ch. 17 - Figure 17.7 You are working in a molecular biology...Ch. 17 - Figure 17.8 Do you think Dolly was a Finn-Dorset...Ch. 17 - Figure 17.15 In 2011, the United States...Ch. 17 - GMOs are created by. generating genomic DNA...Ch. 17 - Gene therapy can be used to introduce foreign DNA...Ch. 17 - Insulin produced by molecular cloning is of pig...Ch. 17 - Bt toxin is considered to be. a gene for modifying...Ch. 17 - The Flavr Savr Tomato: is a variety of...Ch. 17 - generated after a cDNA library is made unique...Ch. 17 - Linkage analysis. is used to create a physical map...
Ch. 17 - Genetic recombination occurs by which process?...Ch. 17 - Individual genetic maps in a given species are:...Ch. 17 - Information obtained by microscopic analysis of...Ch. 17 - The chain termination method of sequencing uses...Ch. 17 - Whole-genome sequencing can be used for advances...Ch. 17 - Sequencing an individual person’s genome is...Ch. 17 - What is the most challenging issue facing genome...Ch. 17 - Genomics can be used in agriculture to generate...Ch. 17 - Genomics cam be used on a personal level to:...Ch. 17 - What is a biomarker? the color coding of different...Ch. 17 - A protein signature is: the path followed by a...Ch. 17 - Describe the precess of Southern blottingCh. 17 - A researcher wants to study cancer cells from a...Ch. 17 - How would a scientist introduce a gene for...Ch. 17 - If you had a chance to get your genome sequenced,...Ch. 17 - Why is so much effort being poured into genome...Ch. 17 - How could a genetic map of the human genome help...Ch. 17 - Explain why metagenomics is probably the most...Ch. 17 - How can genomics be used to predict disease risk...Ch. 17 - How has proteomics been used in cancer detection...Ch. 17 - What is personalized medicine?
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- When the genetic materials are improperly replicated and error cannot be corrected the cell proceeds to:arrow_forwardDescribe the technique of in situ hybridization. Explain how it can be used to map genes.arrow_forwardInformation obtained by microscopic analysis of stained chromosomes is used in: a. radiation hybrid mapping b. sequence mapping c. RFLP mapping d. cytogenetic mappingarrow_forward
- Explain how and why PCR can be used to amplify DNA. Describe the steps in the process. Be sure to address the role of Taq DNA polymerase.arrow_forwardWhat type of experiment would you be most likely to use to demonstrate that protein X was directly phosphorylated by the M-cyclin/CDK pair a flow cytometry experiment a PCR based assay an in vitro assay a fluorescence microscopy experimentarrow_forwardDescribe how each of the following is used DNA hybridization. Polymerase chain reaction (PCR) . DNA sequencing Probe labelingarrow_forward
- Why is fluorescent in situ hybridization (FISH) a useful tool for identifying micrboes? DNA is run over a chip, allowing many different gene to be tested Different probes can show spatial relationships in the cell with other bound probes None of the other choices Probes run across a membrane is a natural follow-up to gel electrophoresis Provides very detailed information about the genetic sequence of the organismarrow_forwardOrder the following technical steps that are required to identify a bacterium by PCR and sequencing of the 16S rRNA gene. [1 mark] - Carry out thermal cycling (amplification) of the PCR - Checking the PCR product (amplication) by agarose gel electrophoresis - Purify the PCR product - Perform a DNA extraction - Sequence the PCR product(Sanger sequencing) - Grow a fresh overnigh liquid culture - Obtain a pure bacterial culture - Set up a 16S Rrna gene PCR - Perform bioinformatic analysis (BLAST)arrow_forwardWhich of the following protocols is BEST used for introducing exogenous DNA into a cell with a thick outer barrier (e.g. plant cell wall)? electroporation O heat-shock transformation gene gun Olipofectionarrow_forward
- Which is a complete list of the ingredients that are essential for PCR? nucleotides, DNA template, Taq polymerase, and plasmids nucleotides, DNA template, DNA ligase, and plasmids nucleotides, DNA template, Taq polymerase, and primers restriction enzymes, DNA template, Taq polymerase, and primers nucleotides, DNA template, DNA ligase, and primersarrow_forwardFor which of the following would not require a nucleic acid probe?a. locating a gene on a chromosomeb. developing a Southern blotc. identifying a microorganism with FISHd. constructing a recombinant plasmidarrow_forwardMost yeast grow suspended in culture, but some yeast grow as a film on the top of a liquid culture. These are called flor yeast. The following Southern blot is looking at the FLO11 gene that is thought to be involved in whether yeast are flor or not. DNA was isolated from four different yeast strains. Strains 1 and 3 are normal while strains 2 and 4 are flor (Fidalgo, 2006). Based on the gene map and Southern results below, what aspect of FLO11 determines whether a strain will be flor or not?arrow_forward
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