Question: I have an unknown bacteria project. I have been given two unknown bacteria. I found out that the two bacteria are "Gram negative Rod" and Gram Positive Cocci". Now, which remaining tests should I perform for this project (in order)? I already performed the Catalase Test, which gave a positive result. Also, I already performed the Oxidase test, which the result was negative. I need to perform 8 more tests, which ones should I do (please state it in order)? Thanks.
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- This is a typical lab exam question. (Problem-solving, uses current topic.)Your TA performed the antibiotic disc experiment using supplies from their research lab. The antibiotic discs had the year 2002 written on their containers.Your TA grew a pure culture of unknown bacteria. They applied the culture to a fresh agar plate and applied the 2002 antibiotic discs to the plate and incubated it for growth. They found that the mystery bacteria species was resistant to all three antibiotic discs that were applied to the plate.Are there any control groups that could be used to confirm or refute this multi-drug resistance? Describe them.Answer the following questions: 1. Why do we pass the smear in the open flame of the bunsen burner 3 times? What do we want to achieve by doing this? 2. Is the size of the bacterial smear on the slide very critical to the analysis? Why or why not? 3. Is the thickness of the bacterial smear on the slide very critical to the analysis? Why or why not? 4. Why do we pass the smear in a gentle stream of water after flooding it with a stain? What do we want to prevent by doing this? 5. Is the duration or time of stain application very critical in simple staining? Why or why not? NOTE: Please try to answer all of the questions asked, i promise to give you a good ratingsQuestion:- Describe control strains used in the clinical microbiology laboratory and explain their maintenance in the laboratory. ( write BY WORD and all steps I need). Introduction Discussion References
- Question 1. Pretend you are performing an Indirect ELISA on patient to detect Streptococcus pyogenes. You did all the steps correctly, except you forgot to wash the tube before adding the substrate. How would this mistake affect your results ? Would you still be able to tell if the patient was positive or negative for S. Pyogenes?A student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 CelciusFactors which can influence antibiotic susceptibility testing (size of zone inhibition) are_________________.
- What will pose an ethical issue in these procedures are performed - sterilization?Below is an MSA plate inoculated with bacteria and grown at 37*C for 2 days. Be pecific and thorough in your answers for parts a and b below. The color of the plate is yellow because b) The presence of bacterial colonies indicates that the bacteria can surviveizzes/00071/take/questions/600381 Regarding Gram staining, what will be the appearance of the Gram-positive bacteria if... All steps are done correctly? The slide is not heat-fixed prior to staining? Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? I Not enough acetone:alcohol was used? Carbol fuschin or safranin was omitted? W [Choose ] [Choose ] Purple Clear Pink Orange [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] 88 22°C
- I’m kinda confused on what to study for the test and what questions are gonna be on there but this PowerPoint but I just want you to explain the microbiology screenshots to me in a way I can clearly understand, I need a tutorGive the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 1. Microscope 2. Colony Counter 3. Autoclave 4. Microbiology incubator 5. Drying oven 6. Refrigerator (microbiology) 7. Bunsen burner/alcohol lamp 8. Candle jar 9. Anaerobic jar 10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet 11. Bacteriologic filters (Seitz, Chamberlain, Berkfield) 12. Petri dish 13. Culture tubes 14. Hanging drop slide 15. Durham’s tube 16. Staining rack 17. Thermostatically controlled water bath 18. Inoculating loop 19. Inoculating needle 20. VialsIn this section you will present the results of all the tests that was done to identify your organism Enterobacter aerogenes (your observation). In the laboratory we tabulated our results and created a chart (ID Matrix). Here you are describing the result of each test (in your own words). Include the name of the Indicator used (if any). Color changes that represent positive/negative results must be mentioned here. Your organism is a Gram-negative organism (Enterobacter aerogenes) You must discuss all the IMViC tests and Hydrogen sulfide test in DETAIL. 1. Indole test 2. Methyl red test 3. Vogues-Proskauer test 4. Citrate Test 5. Hydrogen Sulphide Test