Which of the following functions can be fulfill Form sticky ends to insert genes Create mutations in target DNA Cut RNA Cut sequences at an alternative site Guide CRISPR to the target DNA Cut target DNA
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- Which of the following is a correct statement about CRISPR-Cas-9 gene editing? Group of answer choices A single guide RNA (sgRNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence A single guide RNA (sgRNA) recognizes a genomic region followed by a long sequence palindrome repeat A single guide DNA (sgDNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence PAM sequences induce single stranded breaks that are then repaired by the CAS-9 enzymeWhat is the role of the following molecules in the CRISPR mechanism? * Sequence of 3 nucleotide Not a RNA significant sequence Endonuclease motif that is Host genetic synthesized role in information to match a a binding CRISPR target site mechanism sequence Cas9 guide RNA DNA polymerase PAM Cellular DNA TRNA O O 口 ロ 口 ロロConsider the reagents required to perform CRISPR genome editing. Which molecule specifies the a new sequence that will occur at the target site if genome editing is successful? Cas9 nuclease Cas1 Cas2 tracrRNA crRNA sgRNA donor DNA
- Which of the following types of mutations would be produced by repair of a CRISPR-targeted double stranded break through non-homologous end joining? acquisition of a point mutation specified on a donor DNA template deletion duplication inversionCRISPR/Cas9 can be used in genome editing. Among the following statements, which one is correct? The CRISPR and the Cas9 parts both recognise the DNA target sequence and then recruit an endonuclease for cutting it The Cas9 part recognises the DNA target sequence and the CRISPR part cuts it The CRISPR and the Cas9 parts both recognise and cut the DNA target sequence. The CRISPR part recognises the DNA target sequence and Cas9 cuts it.The pre-mRNA transcript and protein made by several mutant genes were examined. The results are given below. Determine where in the gene a likely mutation lies: the promoter region, exon, intron, cap on mRNA, or ribosome binding site. a. normal-length transcript, normal-length nonfunctional protein b. normal-length transcript, no protein made c. normal-length transcript, normal-length mRNA, short nonfunctional protein d. normal-length transcript, longer mRNA, shorter nonfunctional protein e. transcript never made
- Transcribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’Describe one way in which DNA double stranded break repair that occurs because of X-ray damage differs from that caused by Crispr/Cas9 and one way in which they are similar.Which of the following statements is not true about the guide RNA (see this interactive demonstration for help with this question: https://www.biointeractive.org/classroom-resources/crispr-cas9-mechanism-applications) Group of answer choices it contains a sequence of 20 nucleotides that matches a specific sequence in a cell’s DNA When the guide RNA is combined with Cas9, it will guide Cas9 to the target sequence its target sequence can be almost any sequence as long as it occurs near a PAM motif it is a nuclease, a type of enzyme that cleaves DNA
- Label the following parts of the CRISPR-Cas 9 system. C A B C D Target DNA strand PAM sequence Cas9 protein Single-guide RNAPrimers designing for epitope tagging: Design forward and reverse primers to amplify the following gene with 6×HIS-tag on the N-terminus of the protein. To be cleaved and inserted into the plasmid, add restriction sites for EcoRI and HindIII at 5' and 3'. ATGCTCTCCGCCCTCGCCCGGCCTGTCAGCGCTGCTCTCCGCCGCAGCTTCAGCACCTCAGCC CAGAACAATGCTAAAGTAGCTGTGCTAGGGGCCTCTGGAGGCATCGGGCAGCCACTTTCAC TTCTCCTGAAGAACAGCCCCTTGGTGAGCCGCCTGACCCTCTATGATATCGCGCACACACCC GGAGTGGCCGCAGATCTGAGCCACATCGAGACCAAAGCCGCTGTGAAAGGCTACCTCGGAC CTGAACAGCTGCCTGACTGCCTGAAAGGTTGTGATGTGTAAWith regard to RNA polymerase proofreading ability, which of the following is true? A 3'5' exonuclease fixes all errors in mRNA. There are specialized mRNA repair pathways that remove noncomplementary nuclec No proofreading occurs. RNA backtracking can remove noncomplementary nucleotides. RNA and DNA polymerase proofreading are essentially identical. OOOOO