QUESTION 19 In using a Bradford reagent to measure protein concentrations, a protein sample is diluted 100x then diluted 100x again. The concentration of the diluted sample is found to be 0.605 mg/ml. How much the undiluted sample and buffer in volume (microliter, ul) are needed to create a 1 ml solution containing the protein at 2 mg/ml concentration? O 2 ul protein| 998 ul buffer 49 ul protein | 951 ul buffer O 40 ul protein | 960 ul buffer O 20 ul protein | 980 ul buffer

QUESTION 19 In using a Bradford reagent to measure protein concentrations, a protein sample is diluted 100x then diluted 100x again. The concentration of the diluted sample is found to be 0.605 mg/ml. How much the undiluted sample and buffer in volume (microliter, ul) are needed to create a 1 ml solution containing the protein at 2 mg/ml concentration? O 2 ul protein| 998 ul buffer 49 ul protein | 951 ul buffer O 40 ul protein | 960 ul buffer O 20 ul protein | 980 ul buffer

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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QUESTION 36 SDS-PAGE gels are useful in determining the molecular weights of proteins; however, the molecular weights of (one word = adjective) proteins are usually not reliable. %3D
Question 8 The unique stem-loop structures of the transfer RNA helps the RNA perform its function of joining ribosomal proteins to form the sites for protein synthesis. A True B False
QUESTION 9 Cyanogen bromide cleavage of a peptide results in 3 fragments with the sequence: AGRFM, RS, and AKEM. Proteolysis with trypsin results in the fragments: S, FMAK, EMR, and AGR. The sequence of the peptide is [A]. CS Scanned with CamScanner
QUESTION 9 You are trying to separate five proteins, which are listed below, by gel filtration chromatography. Which of the proteins will elute first from the column? cytochrome c (12 kDa) RNA polymerase (99 kDa) interferon- (34 kDa) hemoglobin (62 kDa) glutamine synthetase (621 kDa) O O O O O
Question 5 Furosemide is a diuretic used in the treatment of congestive heart failure and oedema. 20 tablets of furosemide were powdered and 0.5195 g of powder was weighed out, shaken with 300 mL of NaOH and made up to 500 mL with 0.1 M NaOH. A portion of the extract was filtered and 5 mL of the filtrate was made up to 250 mL with 0.1 M NaOH. The absorbance of the diluted solution was measured at 271 nm. A (1%, 1 cm) value at 271 nm in basic solution Stated content of furosemide per tablet Weight of 20 tablets Weight of tablet powder taken for analysis Absorbance reading at 271 nm From the data below calculate the percentage of stated content in a sample of furosemide tablets. (DGW 2005, 103) = 580 HN 40 mg 1.656 g = 0.5195 g = 0,596 OH CI / NH₂
QUESTION 28 The pK1, pK2, and pKR of the amino acid lysine are 2.2, 9.1, and 10.5, respectively. The pK1, pK2, and pKR of the amino acid arginine are 1.8, 9.0, and 12.5, respectively. A student at SDSU wants to use ion exchange chromatography to separate lysine from arginine. What pH is likely to work best for this separation? 1.5 7.5 10.5 2.5 5.5
Question 15 The GroES subunit has 14 identical 549-residue subunits arranged in two stacked rings with 7 subunits eac True O False
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Question 27 The Whittaker classification is based upon all of the following EXCEPT level of organization habitat © type of cell D type of nutrition O 29 minutes remaining E Past due. This attempt will be submitted late.
Question 19 Micelles are bilayer of lipids that bury their non-polar tails outside the spherical structure in a polar environment. O True False
Question 29 Cationic exchange chromatography - used for positively charged proteins O True False
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