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Provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.
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- Explain the significance of aseptic culture technique in growing microorganisms.Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?discuss the significance of the different aseptic techniques utilized in maintaining sterility of the cell culture lab.
- Describe the difference between batch and continuous culture techniquesExplain why is it important to follow proper precautions and use aseptic technique when working with clinical samples.List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?
- Aseptic technique refers to A) the microbial inoculum placed into a test tube or onto a Petri plate. B) a series of practices to avoid contamination. C) the autoclave and other sterilizing procedures. D) cleanliness in the laboratory.What is the physical appearance (solid, liquid, semi-solid) of following media: agar plateSolid and liquid media are commonly prepared based on manufacturer’s instructions. If no instruction is provided on how the media should be sterilized, what would be the best option? METHOD:RATIONALE:
- Mueller-Hinton agar needs to be made to specific standards in order to yield reliable, reproducible results. How deep is the agar on a Mueller-Hinton agar plate? a) 2 mm b) 4 mm c) 6 mm d) 8 mmWhat are some methods to prevent contamination during streak plating a medium?In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropper