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- List 5 other methods of sterilization and describe the principle/s and when each method is used.
- What are the requirements to support microbial growth?
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- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperOther than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.Make a list of the different types of sterilization, including the method, the principle, the materials that can be sterilized, the required temperature and time, and the required temperature and time. Method Principle Apparatus Temp/Time Materials
- Outline the different physical and chemical methods of sterilization. Briefly describe how each method control microbial growthProvide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.Agar is a solidifying agent used in media preparation. What makes it ideal for cultivation of microbes?
- With the aid of diagrams, explain the processes and principles of each of the following methods: (a) Gel filtration chromatography on Sephadex G50 b.SDS-PAGECompare and contrast antiseptic and disinfectant. What is the hazard of the splattering tendency in flame sterilizing an inoculating loop? Can inoculation loops and needles be sterilized by autoclaving? Explain.Loop with bacterial inoculum was subjected to flame sterilization prior to streaking on agar plate. Effect: Rationale:
- Identify the method of sterilization/ chemical agent described This chemical agent is used as a standard for evaluating new chemical agents. This chemical agent is used as water disinfectant. A phenol-derivative that is less exotic and more potent than phenol. Considered the best antiseptic. Used as prophylaxis for ophthalmia neonatorum.What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tubeOf what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?