What is the main difference between plates before and after centrifugation? What may be the purpose of concentrating the media and perform a second plating
Q: Briefly define culture medium.
A: Microbiology is the branch of science which deals with the study of microorganisms like, bacteria,…
Q: A swab for culture is received from a doctor's office. The staff forgot to mention the source from…
A: Introduction Microbiology is the branch of science that deals with the study of different types of…
Q: What is the purpose of flaming an inoculating loop? How will you know when you have flamed the loop…
A: Hi, since you have posted multiple questions, we will be providing you with answers for the first…
Q: Why don't you get isolated colonies from the pour plate if the broth culture was not diluted first?
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the…
A: Microbial culture is a method of multiplying microbial organisms by letting them reproduce in…
Q: Please answer it correctly for an Upvote. Thank you. •Explain why cfu/unit analyzed is being used to…
A: CFU stands for colony forming unit and is a measurement unit for estimating the concentration of…
Q: In spread plate method, colonies form within the agar and agar surface. In pour plate method,…
A: Introduction Agar media is essential for the study of microorganisms and molecular biology, It is a…
Q: In what instances would the pour plate method be more appropriate than the spread plat method?
A: Pour plate method is a technique that used to enumerate the number of viable cells in a sample.…
Q: Gram stain of Nodule Suspension Describe the morphology of the crushed nodules under microscopes…
A: The bacteria are stained with the gram stain and can be divided into two groups on the basis of the…
Q: When can gelatin be used in culture media preparation? [Answer in not more than 2 sentences
A: GELATIN: Gelatin, often known as gelatine, is a transparent, colourless, and flavourless culinary…
Q: aseptic technique and pure culture technique
A: ASEPTIC TECHNIQUE_ *Inserting PICC lines. *Performing dialysis. *Inserting catheters. *Running…
Q: Describe the bacteria observed in different staining procedures. Please refer to the photo -…
A: Bacteria can be stained by a variety of stains so that they can be visualised in contrast to the the…
Q: What is the difference between the pour plate method and the spread plate method? Why might you…
A: Standard plate count method is a growth based approach that counts the number of living…
Q: In the laboratory, autoclaving is required to prepare media for the growth of What type of method of…
A: The most effective method by which we sterilize the laboratory instruments is known as autoclaving.
Q: Microbiology
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: Can you tell IF the pasteurization process is working in this milk producing plant? How can you…
A: Pasteurization is a process in which milk or milk product is heated to a certain temperature for a…
Q: Can culture media be placed in a freezer? Why or why not
A: Culture media are also called as growth media. They are mixture of nutrients and other substances…
Q: Explain the rationale behind Macrohematocrit test (Wintrobe method) and Microhematocrit test (Clay…
A: Hematocrit test is used to check the proportion of red blood corposcles (RBC) present. If the RBCs…
Q: What is the purpose of the quadrant streak plate method? - How could this method be used in a…
A: In quadrant streak plate method, first of all the plate on which bacteria has to be grown that is on…
Q: How do you sterilize the following? petri dish inoculating loop culture media test tube…
A: Sterilization of following- petri dish - By using autoclave or by a series of steps that includes…
Q: Both the Endospore Stain Protocol and Mannitol Salt Agar Media are differential. How can this be so?
A: The growth media are nutritious media that are used to grow microbes (bacteria and fungus) in the…
Q: What are the purposes of carrying out the streak plate andpour plate techniques in microbiology?
A: It is a technique used to isolate a pure strain from a single species of microorganism, often…
Q: How is the correct way of incubating culture plate media? Explain why is that so.
A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…
Q: Why do you think the continuous culture has higher chances of contamination than batch and fed-batch…
A: Continuous culture may be a set of techniques wont to reproducibly cultivate microorganisms at…
Q: You were given a mixed nutrient agar broth culture of bacteria 1a. How will you determine the…
A: A mixed bacterial culture consists of more than one bacterial cell types growing together. On the…
Q: How is agar in the petri dish prepared to grow the bacterial specimen? explain in your own words.
A: Agar is a polymer made up of subunits of sugar galactose and is a component of cell walls of several…
Q: The students of a Microbiology class were tasked to transfer or subculture a pure culture of…
A:
Q: You have a broth medium that you would like to turn into a solid medium in order to do streak…
A: The "nutrient broth" is essentially nutrient agar without the solidifying agent. They stay liquid at…
Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…
A: Preparation of culture media and inoculation are two of the very sensitive steps in any…
Q: During media preparation, you observed that the agar medium did not solidify after sterilization…
A: Nutrient Agar Medium is the artificial media which is used to grow microbes. It provides essential…
Q: WHAT IS THE TYPE OF Growth patterns on Agar Slant and Broth Medium
A: Growth and morphogenesis of plant is determined by the composition of culture media. There are few…
Q: Which of these microbial structures is the most difficult to kill when attempting to sterilize…
A: Answer of the question given below...
Q: Bacterial cells can form colonies on the following media EXCEPT: Select one: Nutrient broth Agar…
A: The nutrient broth is the liquid nutrient media that lacks the solidifying agent. It is used for…
Q: What is the main advantage of using the slide culture technique for identifying molds?
A: Side Culture - It is a rapid method of preparing fungal colonies for examination and identification.…
Q: Are TSA, MRS and MOX differential media? Why or why not?
A: In microbial biology, differential media refers to any growth media that comprises of certain…
Q: How does an autoclave sterilize instruments? What is used to dry glassware and at the same time
A: Autoclaving is the best method for sterilizing glass instruments. Sterilizing means removal of all…
Q: With liquid media such as broth, is it possible to determine if the culture is pure using the naked…
A: A culture is a method by which microbes are allowed to reproduce and multiply in predetermined…
Q: What is the nature of samples that should NOT be placed inside the drying-oven?
A: According to the question, we have to explain the nature of the samples that should not be placed…
Q: What is the difference between selective and differential media? Give one example of each.
A: Culture medium is referred to as a medium of nutrients that supports microbic growth. The amount of…
Q: What is the major difference between an enrichment culture and a selective culture?
A: Culture media is used for cultivating microorganisms. Both selective media and enriched media are…
Q: Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar…
A: # Here I am giving solution of first two questions . Please repost your third question and better to…
Q: Why does uninoculated agar plates still has visible growth of microorganisms? Where do you think the…
A: Microorganisms are present in abundance in nature. When there are no aseptic conditions then…
Q: The following methods used for sterilizing an antibiotic solution. i. Dry heat sterilization ii.…
A: Sterilization: Microorganisms cause contamination, infection, decay and it is important to remove…
Q: How do you do the continuous culture of bacteria by chemostat and turbidostat
A: Answer: CONTINUOUS CULTURE : It is the method of culturing/growing of bacteria in a media in a…
Q: Can pure culture be prepared directly from a mixed culture? Explain your answer.
A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…
Q: What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube
A: Bacterial culture media is a kind of growth media in which the required bacteria is cultured. The…
Q: What are the primary uses of the following preparations of culture media? Agar regular slant Agar…
A: Hello. Since your question has multiple sub-parts, we will solve the first three sub-parts for you.…
Q: What is this media? What type of media is it? Which type of organism can grow on this plate?…
A: Media is... Agar This is the solids type of Media. Organism type... Streak plate technique is used…
Q: bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows:…
A: CFUs are a measure of the number of progenitors present in a given population of cells; if an…
What is the main difference between plates before and after centrifugation? What may be the purpose of concentrating the media and perform a second plating?
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- In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?What is the best streaking technique in a culture media? ExplainWhat are the possible reasons why culture plates may have too many colonies despite performing serial dilutions and OD600 readings?
- What are the advantages and disadvantages of using the Wet Mount technique? What are the advantages and disadvantages of using the Hanging drop technique? What are the advantages and disadvantages of using the Slide Culture technique? pls elaborate each, thank youWhy does a streaking method used to inoculate plates result in isolated colonies? (Explain systematically)What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?
- Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the pre-made plates by the time of the laboratory schedule?In preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?