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Can a pure culture be prepared from a mixed culture in a broth or plate? Explain.
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- You have a broth medium that you would like to turn into a solid medium in order to do streak plates. What could you add to your media to allow it to solidify? Paragraph В I UWhat is the purpose of adding mineral oil to an O/F Media tube and why do we only put mineral oil in only one? Which sugars are typically tested in the phenol red broth? Can the phenol red test be also used to determine if a certain bacteria can metabolize various carbohydrates? Explain how conducting the MR/VP test too soon in a culture’s growth cycle can lead to false negatives? Generally, the color change we see in these tests is a result of what change in the media? Explain. What is the MR-VP results for coli and E. aerogenes?Serial dilution is recommended prior to pour and spread plate. Why is this so?
- Can the resazurin reduction test be used to determine whether a milk is properly pasteurized or under pasteurized? Explain.Which is more Suitable for long-term storage: agar slant or plate? Explain why?The following ingredient list represents which type of growth media? Beef extract 1.5 g Yeast extract 3.0 g Peptone 6.0 g Glucose 1.0 g Agar 15.0 g water 1000 ml pH 6.6
- Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?Is the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind of media based on what kind of microorganisms it allows to growDemonstrate your understanding of industrial microbiology by explaining the involvement of microorganisms in the following items, mentioning the type of microorganisms involved. AZD1222 vaccine Tempeh (fermented soybean) (i) (ii) (iii) Kombucha
- If cultures are suffering severely due to alkaloid production and accumulation in the medium then, how can i overcome this problem?Below is an image of 3 different bacteria, letter labeld, growing on MacConkey Agar. Note that bacteria such as E. coli use mixed acid fermentation, with very high acid production which will cause a colored precipitation around colonies on MacConkey agar. MacConkey Agar CExplain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium whose components and concentrations are given below. Yeast extract 5 g/L Peptone 10 g/L NaCl 10 g/L Agar 1.5 g/L