Q: What is the BEST glassware that could be used to hold the agar powder while weighing? After 24…
A: As per our guidelines, we are allowed to answer only one question. Kindly upload the rest of the…
Q: Why is dilution a necessary part of pure culture preparation?
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Q: In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside…
A: Several laboratory techniques necessitate smear preparation. A smear can be made using either broth…
Q: Can culture media be sterilized in the hot air sterilizer? Why or why not?
A: Hot air sterilization is a procedure in which the sterilization of the objects are done using hot…
Q: Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled…
A: Agar or agar-agar is a jelly-like substances obtained from red algae. Agar is a solidifying agent…
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Q: Need help Can a pure culture be prepared from a mixed culture in a broth or plate? Explain.
A: INTRODUCTION How to make a pure culture from mixed culture is explained below.
Q: What is the difference between the pour plate method and the spread plate method? Why might you…
A: Standard plate count method is a growth based approach that counts the number of living…
Q: which of the following is best to sterilize heat-liable (sensitive) solutions? a, pasteurization…
A: From above question Option d (filtration) is correct.
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A: Optical tweezers have been used to trap dielectric spheres, viruses, bacteria, living cells,…
Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…
A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…
Q: Serial dilution is recommended prior to pour and spread plate. Why is this so?
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Q: Can culture media be placed in a freezer? Why or why not
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Q: Why is it important to use a sterilized loop between streaks when preparinga streakplate?
A: Streaking is a technique used to isolate a pure strain from a single species of microorganism, often…
Q: What advantage(s) does the pour plate method have over the streak plate method?
A: In microbiology. streaking is a technique for the isolation of the pure strain from the population.…
Q: yield
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Q: Why is it important that the agarose is exposed to ethidium bromide, which is either added to the…
A: Ethidium bromide is utilized during separation of fragments of DNA by agarose gel electrophoresis.…
Q: You have a broth medium that you would like to turn into a solid medium in order to do streak…
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Q: Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation?
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Q: what is oil immersion and why is it used
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Q: chemically defined media b. complex culture media
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Q: What is the purpose of adding sodium bicarbonate to a culture medium?
A: >> The purpose of adding sodium bicarbonate to animal culture medium is that it acts as a…
Q: If a component is heat sensitive, how might you conveniently achieve sterility of that medium? a.…
A: Option A i.e. filtration
Q: Can culture media be sterilized in the hot air oven? why or why not?
A: Sterilization is the process of deactivating, killing, or removing any kind of microorganism (or…
Q: With liquid media such as broth, is it possible to determine if the culture is pure using the naked…
A: A culture is a method by which microbes are allowed to reproduce and multiply in predetermined…
Q: Why do we add powdered zinc to the nitrate reduction test?
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Q: What is the nature of samples that should NOT be placed inside the drying-oven?
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Q: What is the best streaking technique in a culture media? Explain
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Q: How much liquid media should you put in your dilution blank if you wanted to serially dilute a1 ml…
A: Serial dilution of the original sample containing microorganisms to achieve countable colonies on…
Q: What type of agar is MacConkey agar?
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Q: Aside from Paraffin, what is your recommended infiltrating medium for routine purposes? why?
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Q: What is diluent? Why is it useful for working with bacterial samples?
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Q: What is the hazard of the spattering tendency in heating
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Q: How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long…
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Q: Agar is a solidifying agent used in media preparation. How and why does the agar concentration in…
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Q: What is the basic purpose of the paper chromatography?
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Q: How would you inoculate a plate to get a 1:10 dilution? A 1:100 dilution?
A: Inoculation is defined as the process of introduction of microorganisms into a culture for their…
What is one advantage of a pour plate over a streak plate?
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- What advantage(s) does the pour plate method have over the streak plate method?What is the difference between the pour plate method and the spread plate method? Why might you choose one over the other? What kind of plate must this be and why do you say that?What are some methods to prevent contamination during streak plating a medium?
- What are pure cultures and why are they important? How are spread plates, streak plates, and pour plates prepared?For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise when the slide is heated in a flame?A culture medium is comprised of the following components: 0.5% peptone, 0.3% meat extract, 1.5% agar, and distilled water. If you are asked to prepare 765 mL of culture media, how much meat extract would you need?
- What is the basic purpose of the paper chromatography?What is the physical appearance (solid, liquid, semi-solid) of following media: agar plateTo prevent inclusion from occurring during precipitation, the technique to follow is I. filter precipitate with less porous paper II. crystal growth be made to occur more slowly which of the two is correct?
- Salmonella-Shigella (SS) agar plate results (kitchen sink drain and kitchen dish cloth) Using the following information about SS agar and the image below, describe and identify the growth on the SS agar plate in the table on page 6. SS agar plate - results of kitchen sink drain and kitchen dish cloth (plate below) Salmonella-Shigella (SS) agar: Salmonella-Shigella agar (SS agar) is a selective and differential medium utilized for the selection and characterization pathogenic enteric bacteria. SS agar contains lactose, bile salts, ferric citrate, and neutral red. Kitchen The bile salts select for Gram-negative bacteria, while inhibiting Gram-positive bacteria. sink drain The other components differentiate among Gram-negative bacteria. Coliform bacteria such as E. coli will ferment the lactose in the media, resulting in bacterial growth with a pink color. (They do not produce any hydrogen sulfide.) Members of the genus Salmonella will not ferment lactose, but do product hydrogen sulfide…Design a serial dilution of a 25-gram cheese sample to achieve a final dilution of 10-5 using 90 ml blanks. Show how you would plate out 10-2 through to 10-6what is the effect and rationale when three milliliters instead of 1 mL was placed in the Petri dish in pour-plate method?