Please explain the differences between freeze drying,drum drying and air drying and which give a conclusion on which drying method is the best in removal of water in microbial cells.
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Please explain the differences between freeze drying,drum drying and air drying and which give a conclusion on which drying method is the best in removal of water in microbial cells.
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- Provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.EMB agar inhibits the growth of Gram-positive bacteria while encouraging the growth of Gram-negative bacteria. It also can determine if some bacteria use lactose. It is particularly effective at determining if E. coli is present in a specimen. Define what type(s) of media EMB agar is, using as many terms in the answer list as are applicable.Why is a Bunsen burner used in the microbiology lab? (select all that apply) The flame is used to sterilize the inoculating loop. The flame kills microorganisms that float in the air and could contminate samples. To sterilize the lid of the agar plate. To reduce the spread of antibiotic resistance.
- Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayWhy is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.
- What is the physical appearance (solid, liquid, semi-solid) of following media: agar platewhich method do you think is more reilable, a test utilizing microbial growth media, or a biochemical test? explain your answer.In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down. For "autoclaving" For Dry heat oven Can be sterilized only sterilization with either Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-ml graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-ml beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropper
- Make a list of the different types of sterilization, including the method, the principle, the materials that can be sterilized, the required temperature and time, and the required temperature and time. Method Principle Apparatus Temp/Time MaterialsWhat are some methods to prevent contamination during streak plating a medium?Describe the step by step method of preparing culture media in microbiology.