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- Provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000What could be possible errors when you incorrectly identify an Unknown organism when using IMViC tests in Microbiology?
- discuss the significance of the different aseptic techniques utilized in maintaining sterility of the cell culture lab.Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple sugar iron agar test.
- For the pour plate method, state which plate will be chosen for colony isolation and why? From the chosen plate, indicate (circle) the colonies for both bacterial species that will be selected and transferred to new nutrient agars as a pure culture.Which type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus pyogenes from a patient’s throat swabb. isolating a pathogen from a patient’s clean-catch urine samplec. isolating enteric bacteria such as Escherichia coli from a sample oforganically grown spinach d. maintaining a patient’s nasal swab specimen for further analysis andidentifi cation of possible respiratory syncytial virus (RSV) infectionDiscuss fresh, living preparations vs fixed preparations. What are the advantages and disadvantages of each technique in a microbiology laboratory?
- Which one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? Select one: a. Agar macrodilution method b. Gradient diffusion method c. Broth microdilution method d. Disk diffusion methodexplain how the following genotypic andphenotypic methods of microbe identification is done. Use diagrams or schematic representations to explain yourwork. 1.Gram Staining2.Biochemical Reactions: Multiple Tube Fermentation Technique3.(Biochemical Reactions: IMViC TestWhat is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of an E-test strip.