Pre-Lab Questions 1. Define a protein, and describe one important function of proteins in the human body. 2. The following data were obtained from a Bradford assay to determine the protein content in a sample: Protein content (ug) Absorbance value 0 0.000 2 0.022 5 0.065 10 0.106 20 0.178 30 0.299 40 0.380 50 0.472 unknown 0.150 Use Excel, another program, or graph paper to produce a calibration curve based on the data. Use linear regression (or best fit line) to obtain the slope of the line. Determine the unknown protein content for a sample that shows an absorbance reading of b. С. 0.150.

Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
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Pre-Lab Questions
1. Define a protein, and describe one important function of proteins in the human body.
2. The following data were obtained from a Bradford assay to determine the protein content in a
sample:
Protein content (ug)
Absorbance value
0
0.000
2
0.022
5
0.065
10
0.106
20
0.178
30
0.299
40
0.380
50
0.472
unknown
0.150
Use Excel, another program, or graph paper to produce a calibration curve based on the data.
Use linear regression (or best fit line) to obtain the slope of the line.
Determine the unknown protein content for a sample that shows an absorbance reading of
b.
С.
0.150.
Transcribed Image Text:Pre-Lab Questions 1. Define a protein, and describe one important function of proteins in the human body. 2. The following data were obtained from a Bradford assay to determine the protein content in a sample: Protein content (ug) Absorbance value 0 0.000 2 0.022 5 0.065 10 0.106 20 0.178 30 0.299 40 0.380 50 0.472 unknown 0.150 Use Excel, another program, or graph paper to produce a calibration curve based on the data. Use linear regression (or best fit line) to obtain the slope of the line. Determine the unknown protein content for a sample that shows an absorbance reading of b. С. 0.150.
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