Item 29: Question What is the relative retention (a) for compounds A and B if the retention time for A (TA) is 3.91 min, the retention time for B (tg) is 6.42 min, and the tm is 1.34 min? 0.51 1.64 1.98 O 3.79
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29. What exactly is relative retention and retention time? How do you solve problems like this?
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- This is a Biochemistry lab question. For the lab we are spectrophotometrically determining the pka of PNP based on different pHs. I have a sigmoid graph of the absborances of PNP at different pHs at 400 nm. I have pH on the x-axis and absorbance on the y-axis. How do a calculate the pka based off of this graph?Dr. Waybell designs a study to investigate whether the dose of melatonin taken before bed impacts people's moods. Dr. Waybell rejects Ho and computes 7²=0.05. She has made a Type Il error. O Dr. Waybell retain reject Ho and computes n=0.005. She has made a Type Il error. O Dr. Waybell rejects Ho and computes 7=0.005. She has made a Type Il error. Dr. Waybell retain reject Ho and computes n=0.05. She has made a Type Il error.What value of absorbance corresponds to 45.0% T?
- Question 2 Zinc was analysed in acid mine drainage (AMD). The signal obtained from an AAS for the sample was 0.125 absorbance units. 25.00 mL of a 0.15 ppm Zn standard was added to 75.00 mL of the sample. The signal obtained was 0.750. a. The 0.15 M Zn standard of volume 500 mL was prepared from zinc (II) phosphate. Determine the mass of salt required to make the standard. 4.43 x 10 g -4 b. Determine the Zn concentration in the AMD sample. [Zn] = 0.00619 ppmGive a clear explanation handwritten answer...give handwritten answerThe concentration of methylene blue in the sample is estimated through (select the correct) options: refractive index measurement (refractometry). turbidity measurement (turbidimetry). absorbance measurement (spectrophotometry). titration with 2% HCl solution.
- How does the retention time of a compound change with separation conditions? Is this change linear (e.g., all compounds respond to the same extent)?0.200 M 0.00200 M Test Tube No. [FESCN2*1std (M) Absorbance Fe(NO3)3 (mL) KSCN (mL) 17.98 1.97 0.815 5 (Standard) Edit Edit Edit NG 1 Info▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min, and IS at 4 min. In the patient sample, the analyte elutes t 2 min and the IS at 8 min. What is the relative retention time and the analyte in the patient? A) methanol B) Ethanol chu C) IPA
- 3) What is the approximate retention time of benzoate?5. (1) (ii) Ibuprofen (206.3 g/mol) absorbs at 255 nm and shows a molar absorptivity of 3010 L/mol.cm. A tablet of ibuprofen, 0.6654 g, was ground and 0.2203 g powder was dissolved in 20% methanol in a 100 mL volumetric flask (Solution A). Exactly 5.0 mL of Solution A was diluted in a 50.0 mL flask and the resulting solution showed an absorbance of 0.1228 AU in a 1.00 cm cell. (i) What is the concentration (mol/L) of ibuprofen in the 100 mL stock solution? (ii) Report the amount of ibuprofen in mg/Tablet. Ibuprofen H3C | HCCH; 1 H3C CH3 -CH-COOH1) Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. STAAT 61 1.11 227 RT TYPE AREA XXXX XXXX XXXX AREAS 0.009 55874 44.117 ARIHT 0.61 XX XX XX 1.11 2.27 XX XX XX TOTAL AREA=XX MUL FACTOR=XX 1. What is the retention time of compound A? Compound B? 2. Which compound is present in a larger amount? 3. Which compound has the lower boiling point? 4. What would happen to the retention times of compounds A and B if the column temperature were raised? 5. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?