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Can culture media be sterilized in the hot air sterilizer? Why or why not? (Answer in not more than 5 sentences)
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- Why is heat a highly effective sterilizing agent? (Answer in not more than 2 sentences)Which physical or chemical agent would be the best choice for sterilizing the following items: glass pipettes, tryptic soy broth tubes, nutrient agar, antibiotic solution, interior of a biological safety cabinet, wrapped package of plastic Petri plates? Explain your choices.Why is 15 minutes generally used for sterilization? Why is agar added to medium?
- Other than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.What will pose an ethical issue in these procedures are performed - sterilization?List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?
- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperWhat is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tubeSolid and liquid media are commonly prepared based on manufacturer’s instructions. If no instruction is provided on how the media should be sterilized, what would be the best option? METHOD:RATIONALE:
- Match the most appropriate method of sterilisation for the following items. 10 mL vitamin solution 5 mL antibiotic solution Petri dishes 1 L of water 1 L of nutrient broth medium Talcum powder Charcoal powder [Choose ] [Choose ] Gamma radiation Autoclave Dry heat oven Filtration [Choose ] [Choose ] [Choose ] [Choose ] [Choose ]Define the following terms; Sterilization, and Aseptic Technique. What are the differences between the two concepts?Kligler’s iron agar and SIM are multiple test media.. Both media are stabbed but for different reasons. Explain.