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- Alternative name for Moist-heat sterilization Select one: Oven sterilization Autoclaving Ionizing radiationdescribe the 3 processes that use moist heat methods specifying time and temperature for each: pasteurization autoclaving tyndallizationIdentify the method of sterilization/ chemical agent described At 15psi, the temperature reaches 121 degree Celsius requiring only 15 to 20 minutes to sterilize the material. The material to be sterilized is exposed to live steam for 30 minutes for 3 consecutive days.
- Disinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%Serial dilution is recommended prior to pour and spread plate. Why is this so?Can culture media be sterilized in the hot air sterilizer? Why or why not? (Answer in not more than 5 sentences)
- You are given reference temperature 121 °C, Z value 10 °C, F0 15 min for killing Clostridium botulinum in commercial sterilization. Calculate F value when sterilized at 131 °C in canning processing.Match the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungiFor preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise when the slide is heated in a flame?
- Which physical or chemical agent would be the best choice for sterilizing the following items: glass pipettes, tryptic soy broth tubes, nutrient agar, antibiotic solution, interior of a biological safety cabinet, wrapped package of plastic Petri plates? Explain your choices.In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down. For "autoclaving" For Dry heat oven Can be sterilized only sterilization with either Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-ml graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-ml beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperGive two reasons for heating the slide after the smear is air dried?