After the mordant has been added, Gram-positive organisms are stained __________ and Gram-negative organisms are stained __________. A)purple;pink B)purple;purple C)purple;colorless D) pink;pink
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After the mordant has been added, Gram-positive organisms are stained __________ and Gram-negative organisms are stained __________.
A)purple;pink
B)purple;purple
C)purple;colorless
D) pink;pink
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- The sediment observed in stained blood smear caused by:a) Overstrainingb) More Haematoxylin in the mixturec) Smear was not fixedd) Using non- filtered staine) Using old stainColony formation can be observed in liquid media. a) Trueb) FalseIf you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would you expect? a) gram negative bacteria would stain purple b) gram negative bacteria would be unstained c) gram positive bacteria would stain pink d) gram negative and gram positive would be unstained
- The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization?3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distilleList one method of disinfection, and one method of sterilization. Label which is which. A/Which of the following is used in the sterilization of the medium?a) benmargeb) swabc) autoclaved) centrifugee) cooling
- You are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. 6) What would be the last reagent you use to identify the bacteria? 7) What type of visual aid you need to help you see the bacterial samples clearly? 8) After the use of what reagent, you will be able to decide if you have a Gram positive or Gram negative bacteria? **Please give the answer**1. The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization? 3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distilledDescribe the microscopic morphology of each sample observed with a Gram stain (S. aureus, P. aeruginosa, B. subtilis and dental plaque). Indicate the Gram reaction of each.
- Filtration does/is NOT...?a) Sterilize heat sensitive liquidsb) Kill microbesc) Sterilize providing pores are exceedingly smalld) Used in preparation of vaccines, vitamins, and drugse) Used to capture microbes in ventilation systems via HEPA filters Which of the following is false?a) Pasteurization does not sterilizeb) Ionization radiation is useful for sterilizing food and pharmaceuticals c) Phenolics can be used as antisepticsd) Isopropyl alcohol can sterilize the skine) Steam autoclaving is an effective means of sterilization Which of the following is not a factor that determines the effectiveness of an antimicrobial agent? a) Time of exposure b) Temperature c) pH d) Concentration of agent e) All of the above are factors that determines the effectiveness of an antimicrobial agent 4. Which of the following is not a specific cell target for antimicrobial agents? a) Capsule b) Cell wallc) Cell membraned) DNA / RNA synthesis e) Protein function 5. Which of the following…If the pH indicator were left out of MacConkey agar, the medium would bea) complex.b) differential.c) defined.d) defined and differential.e) complex and differential.How do you observe growth in liquid microbiological media? a) the medium is cloudy or turbid b) colonies appear on the surface of the medium c) the medium becomes solid d) colonies appear distributes throughout the medium