13. In bacteria, mismatch repair involves: A. DNA glycosylase/lyase B. AP endonuclease C. RecA and LexA proteins D. MutS, MutL and MutH proteins E. UvrABC proteins 14. _______ involves an exchange of DNA between two interacting DNA molecules. A. Transference B. Transposition C. Crossing over D. Induction E. None of the above 15. The bacterial core polymerase has two functions: 5' to 3' polymerase activity and _________________ activity. A. 5' to 3' exonuclease B. 3' to 5' polymerase C. 3' to 5' exonuclease D. topoisomerase E. none of the above

13. In bacteria, mismatch repair involves: A. DNA glycosylase/lyase B. AP endonuclease C. RecA and LexA proteins D. MutS, MutL and MutH proteins E. UvrABC proteins 14. _ involves an exchange of DNA between two interacting DNA molecules. A. Transference B. Transposition C. Crossing over D. Induction E. None of the above 15. The bacterial core polymerase has two functions: 5' to 3' polymerase activity and ___________ activity. A. 5' to 3' exonuclease B. 3' to 5' polymerase C. 3' to 5' exonuclease D. topoisomerase E. none of the above

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section: Chapter Questions

Problem 3TYK: Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning...

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10. _E.coli phage T4 DNA is terminally redundant and ____________________.A. linearly permutedB. circularly permutedC. single-strandedD. blunt endedE. none of the above 11. ___________ allows cells to maintain the chromosome number.A. MeiosisB. MitosisC. Binary fusionD. BuddingE. None of the above 12. ___ is the repair of UV-damaged DNA by a light-dependent enzymatic reaction.A. PhotoreactivationB. Sensory deprivationC. PhotoinductionD. PhotosynthesisE. none of the above
Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.
16. Alu elements in the human genomes are examples of:A. SINE elementsB. LINE elementsC. Autonomous transposable elementsD. Composite transposonsE. Noncomposite transposons 17. The pol gene of a typical retrovirus encodes:A. A reverse transcriptaseB. A DNA polymeraseC. An integrationD. A proteaseE. All but one of the above
Q: Antibiotics are used in genetic engineering. They are useful to keep culture free of microbial a O infections O to select healthy vectors .b O to identify replication start sites.c O as selectable markers .d Q: Choose the best choice that describes the purpose of adding chloramphenicol to the culture of engineered bacteria O It inhabits the process of protein a synthesis O It blocks chromosome replication .b It blocks cell division .c It leads to increase the copy number of d recombinant molecules Q: Alkaline denaturation process is a kind of DNA isolation on the basis of conformation, that cause insolubility of cell components except one of the following Supercoiled DNA a Chromosomal DNA b Protein molecules c RNA molecules d All choices.e None of choices f Q: After transforming the ligation mixture into the E. coli, the transformed and non- transformed bacteria both can grow on media .containing antibiotics True a O False ..b
11. What is the phenotype of the bacterial strain with the following lac genotype: IPOʻZ* Y A/P P* o* Z* Y* A a. lacZ is constitutive, lacY is inducible, lacA is not expressed b. lacZ is inducible, lacY is inducible, lacA is not expressed c. lacZ is constitutive, lacY is not expressed, lacA is not expressed d. lacz is inducible, lacY is not expressed, lacA is not expressed e. lacZ is not expressed, lacY is not expressed, lacA is not expressed
7. Replication factor C plays which of the following roles in eukaryotic DNA replication?A. the sliding clampB. the clamp loaderC. the DNA ligaseD. a single-stranded DNA binding proteinE. a cofactor required for activating the DNA polymerase 8. Phages that program the host cell for continued particle releasing without killing the cell are called ____________ phages.A. lyticB. lysogenicC. temperateD. chronicE. none of the above 9. Retroviruses use _______ to make a DNA copy of their RNA genome.A. restriction enzymesB. DNA polymerase IC. RNA polymerase IID. reverse transferaseE. reverse transcriptase
4. Some transposases use extensive DNA replication to leave one copy of the element behind in a process called ____________________.A. persistent transpositionB. chronic transpositionC. exchange transpositionD. replicative transpositionE. none of the above 5. Which of the following is not an example of a transposable element found in bacteria?A. Tn5B. Tn10C. IS1D. IS903E. Xis 6. Besides a transposase, many common composite transposons in bacteria encode genes for:A. Toxin productionB. Antibiotic resistanceC. DNA repairD. Homologous recombinationE. None of the above
Which of the following gene transfer mechanisms is occurred by cell-to-cell contact? Lütfen birini seçin: O a. Composition O b. Transformation O c. Conjugation O d. Regeneration O e. Transduction
2. Compare and Contrast the horizontal gene transfer mechanisms of Transformation, Generalized Transduction, and HFR Conjugation. Identify the basic steps of each process and identify each mechanism's unique features. A. Transformation: 1. Basic process 2. Unique features: B. Generalized Transduction: 1. Basic process 2. Unique features: cy bluow bout C. HFR Conjugation: 1. Basic process 2. Unique features: lotno lanolighaans T eviliz09.A shular ano
Q: Hyperglycosylated genetically engineered proteins synthesized by some micro-organisms are usually induce human immune system O True a O False .b Q: Find the incorrect statement about plasmids CO they are circular a they replicate independently .b they are transferrable .c they are single stranded .d Q: Discontinuities of DNA nick can be fixed by ........... activity Nuclease .a Modifying enzymes .b O Ligase .c O Polymerisation d Q: Frameshifts means missreading of right sequence of a inserted genes presence of mutation that disturb the b sequence order a process that leads to generate .c different protein sequence All choice .d none of choices.e Q: Despite some drawbacks, E. Coli remains the most frequently used microbial eukaryote for recombinant protein synthesis O True .a O False .b Q: Even when the recombinant proteins are produced in mammalian cells, there are still some drawbacks can be occurred such as Protein folding a The yield of recombinant protein b Recombinant Protein…
slation is synthesis of proteins from B. C. D. t-RNA m-RNA r-RNA DNA 41. It is a type of mutation which modifies the whole sequence of the amino acids. A. В. C. D. 42. Which of the following is a result of a genetic mutation? Inversion Additions Substitutions Replication A. Kwashiorkor Scurvy Cystic Fibrosis Marasmus В. С. D. 43. Which of the following medical condition is a result of a substitution mutation? A. Sickle cell anemia B. Tay-sach's disease C. Cystic Fibrosis D. Some Cancer 44. Lung cancer is caused by which of the following factors? A. В. Addition Inversion Substitution Polycyclic Aromatic Hydrocarbons С. D. 45. Which of the following are called mutagens А. Chemicals that induce changes in DNA В. Chemicals that induce changes in Proteins. C. Chemicals that induce changes in Carbohydrates. D. Chemicals that induce changes in Lipids. 46. Glycolyis is under of the following metabolic pathway? Chain reaction Chemical reaction Anabolic reaction Catabolic reaction А. В. С. D.…
Section two. Match horizontal gene transfer mechanism with best definition. Answers are used more than once. а. Transduction b. Transformation Conjugation c. 44. requires two live cells, where the recipient receives donor DNA 45. involves a virus 46. usually a plasmid is transferred 47. does not require a virus or another cell 48. "sex" for bacteria 49. occurs because of mistake made in chromos ome packaging by a virus 50. Frederick Griffith coined this term.