Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Textbook Question
Chapter 18, Problem 3TYK
Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors?
a. The plasmid must have resistance to accept DNA inserts.
b. They allow the detection of plasmids that contain an inserted DNA fragment.
c. They ensure the presence of the ori site.
d. They ensure that the plasmid can be cut by a restriction enzyme.
e. They allow identification of bacteria that have taken up a plasmid.
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What are the sticky ends of the restriction fragments?
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a. The surfaces of sticky ends contain matching base pairs, allowing fragments to splice.
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B.) What are the requirments of an expression vector.
Chapter 18 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 18.1 - What features do restriction enzymes have in...Ch. 18.1 - Prob. 2SBCh. 18.1 - What information and materials are needed to...Ch. 18.2 - What is a transgenic organism?Ch. 18.2 - Prob. 2SBCh. 18.3 - What is a restriction fragment length polymorphism...Ch. 18.3 - Prob. 2SBCh. 18.3 - Prob. 3SBCh. 18 - Prob. 1TYKCh. 18 - Prob. 2TYK
Ch. 18 - Why are antibiotic resistance markers such as ampR...Ch. 18 - After a polymerase chain reaction (PCR), agarose...Ch. 18 - A cDNA and a cloned fragment of genomic DNA share...Ch. 18 - Prob. 6TYKCh. 18 - Which of the following is not true of somatic cell...Ch. 18 - Prob. 8TYKCh. 18 - Prob. 9TYKCh. 18 - Prob. 10TYKCh. 18 - Prob. 11TYKCh. 18 - Discuss Concepts A forensic scientist obtained a...Ch. 18 - 13. Suppose a biotechnology company has developed...Ch. 18 - Prob. 14TYKCh. 18 - You learned in the chapter that an STR locus is a...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Describe What are the sticky ends of the restriction fragments? Select one: a. The surfaces of sticky ends contain matching base pairs, allowing fragments to splice. b. The surfaces of sticky ends have glue like substance that allow fragments to splice. c. The surfaces of sticky ends contain the exact same nucleotides, allowing fragments to bond. d. The surfaces of sticky ends have velcro like structure, allowing fragments to bond.arrow_forwardWhat is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic acids at specific sites b. to join nucleotides during transcription c. to add new nucleotides to the growing strand of DNA d. to repair breaks in sugar - phosphate backbonesarrow_forwardA plasmid has two restriction cut sites for EcoRI and one restriction cut site for HindIII. The plasmid is subjected to PCR, and the region containing the three restriction cut sites is amplified, such that all three sites are present in the resulting amplicon. If the amplicon is incubated with EcoRI, how many fragments of DNA are expected to result from this digest experiment? a. 0 b. 1 c. 2 d. 3 e. 4arrow_forward
- Plasmids are important in biotechnology because they are Group of answer choices a. a vehicle for the insertion of foreign genes into bacteria b. recognition sites on recombinant DNA strands c. surfaces for protein synthesis in eukaryotic recombinants d. viruses incorporated into the host DNAarrow_forwardChoose the statement that best describes the outcome of transfecting cells with a plasmid that includes the genetic promoter of the hypothetical X protein in a plasmid coding for GFP. A.GFP will be expressed under the same conditions and in the same cells as the X protein. B.GFP combined with the X protein will be expressed in all transfected cells. C.PFM will not be expressed as it requires its own sponsor, the PFM sponsor, to be properly expressed. D.GFP will replace the X protein in transfected cells.arrow_forwardPlasmids are very important for recombinant DNA work. 1.) Describe the components that are important to a cloning vector.arrow_forward
- A geneticist uses a plasmid for cloning that has the lacZ gene and a gene that confers resistance to penicillin. The geneticist inserts a piece of foreign DNA into a restriction site that is located within the lacZ gene and uses the plasmid to transform bacteria. Explain how the geneticist can identify bacteria that contain a copy of a plasmid with the foreign DNA.arrow_forwardWith the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining different stages of the cloning process that involves the following:a. Isolation of target DNA fragments (often referred to as inserts)b. Ligation of inserts into the plasmid, creating recombinant molecules c. Transformation of recombinant plasmids into bacteria or other suitable host for propagationd. Screening/selection of hosts containing the intended recombinant plasmid. For this stage(d), discuss the importance of a second marker that can be used for screening of genomic DNA for colonies containing the pka-1 under the principle of insertional inactivation. This should be properly explained using all the attributes of the plasmid described above.arrow_forwardA linear plasmid contains only two genes, which are transcribed in opposite directions, each one from the end, toward the center of the plasmid. Draw diagrams ofa. the plasmid DNA, showing the 5′ and 3′ ends of the nucleotide strands.b. the template strand for each gene.c. the positions of the transcription-initiation sites.d. the transcripts, showing the 5′ and 3′ ends.arrow_forward
- Why do prokaryotes produce restriction enzymes? A. Restriction enzymes are produced to enable bacteria to repair lesions in its DNA B. Restriction enzymes enable prokaryotes to assimilate genes or fragments of DNA from its environment C. Restriction enzymes excise phage DNA from prokaryotes thereby protecting them from lysis. D. Restriction enzymes help unwind DNA and facilitate DNA replication in prokaryotes E. Restriction enzymes are necessary for editing DNA during replication.arrow_forwardExplain why the genes whose products are required for the normal growth of bacteria not carried on plasmids? Give two examples (one bacterial gene and one plasmid gene) to support your answer.arrow_forwardWhen E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as : A. Ligation. B. Transformation. C. Transfection. D. Digestion.arrow_forward
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