Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 5, Problem 1CT
Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the Stain affect the staining of bacteria?
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Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain affect
the staining of bacteria?
Can dyes other than methylene blue be used for direct staining? Briefly explain.
For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts).
Stain
Cationic or Anionic Dye
malachite green
crystal violet
nigrosin
safranin
Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?
Chapter 5 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 5 - Of what value is a simple stain?Ch. 5 - What is the purpose of heat-fixing the smear?Ch. 5 - Another method of fixing smears is to use methanol...Ch. 5 - In heat-fixing, what would happen if too much heat...Ch. 5 - Methylene blue can be prepared as a basic stain or...Ch. 5 - Prob. 2CTCh. 5 - Bacteria can be seen without staining. Why, then,...Ch. 5 - Quality control staff in a sterilization unit of a...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Is it possible to perform double or triple staining using fluorescent dyes? Explain.arrow_forwardWhy are basic dyes more effective for bacterial staining than acidic dyes?arrow_forwardBoth crystal violet and safranin are basic stains and may be used to do simple stains on Gram positive and negative cells. This being the case, explain how they end up staining Gram positive and gram negative cells differently.arrow_forward
- Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosinarrow_forwardWhat is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain, what is the mordant?arrow_forwardWhat is the difference between simple and differential stains? Give an example of a differential stain.arrow_forward
- Draw what you would expect to see for acid fast stain and endospore stain procedure if you did not stain with counterstain. Color pencils need to be used.arrow_forwardFor What purpose is Giemsa stain used for?arrow_forwardExplain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayarrow_forward
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