Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 5, Problem 1CA
Bacteria can be seen without staining. Why, then, was Koch’s recommendation of fixing and staining important for the discovery of the bacterial causes of diseases?
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Why bacteria require staining before microscopic examination?
Attached below is the study about the effects of handwashing and the effectiveness of soap against bacteria. What conclusions can be generated from this result?
A
C
before washing hands
10 second hand wash
B
D
hot water only rinse
30 second hand wash
Figure 8: Agar plates with bacteria A) before washing hands B) after rinsing hands in hot water C) After washing
hands with soap for 10 seconds and D) after washing hands for 30 seconds. Images show that the number of
bacteria on the plate are not greatly reduced after a hot water rinse or 10 second handwash but bacterial levels are
reduced after a proper hand wash for 30 seconds.
Jessica placed crystal violet ,grama's iodine and safranin reagents on her bench.Which staining procedure is she preparing for with these reagents?
a)Grama's staining procedure
b)simple staining procedure
c)Acid -fast staining procedure
d)Non -acid fast staining procedure
e)Scaeffer -Fulton staining procedure
Chapter 5 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 5 - Of what value is a simple stain?Ch. 5 - What is the purpose of heat-fixing the smear?Ch. 5 - Another method of fixing smears is to use methanol...Ch. 5 - In heat-fixing, what would happen if too much heat...Ch. 5 - Methylene blue can be prepared as a basic stain or...Ch. 5 - Prob. 2CTCh. 5 - Bacteria can be seen without staining. Why, then,...Ch. 5 - Quality control staff in a sterilization unit of a...
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- Koch's postulates include all of the following EXCEPT O 1) Isolate the organism in pure culture. The symptoms of the test animal may vary from the symptoms seen in the O 2) patient. O 3) Inoculate a test animal with the isolated organism. 4) The organism must be isolated from the test animal in pure culture. 5) The organism isolated from the test animal must match the organism isolated from the patient.arrow_forwardAttached below is the study about the effects of handwashing and the effectiveness of soap against bacteria. What conclusions can be generated from this result? (see rubrics for the point marking) A before washing hands C 10 second hand wash B D hot water only rinse 30 second hand wash Figure 8: Agar plates with bacteria A) before washing hands B) after rinsing hands in hot water C) After washing hands with soap for 10 seconds and D) after washing hands for 30 seconds. Images show that the number of bacteria on the plate are not greatly reduced after a hot water rinse or 10 second handwash but bacterial levels are reduced after a proper hand wash for 30 seconds.arrow_forwardIf you conducted an acid-fast stain on a sputum sample from a person with tuberculosis, what would you expect to see on the slide?arrow_forward
- What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method?arrow_forwardWhat is Ziehl Neelsen bacterial staining technique, and what is the target organisms and the purpose of its application? LONG ANSWER NEEDED, DETAILED. Thank you i’ll rate.arrow_forwardWhy is it Koch's postulates cannot be used for the microbe E. Coli. What limitations does E.coli have?arrow_forward
- Blood is stained with stain. a) Methylene blue b) Safranin c) Leishman stain d) Carbol fuchsinearrow_forwardwhat is the purpose of using oil immersion with the 100x objective lens? a)To prevent refraction of light b)To prevent scratching the slide c)To allow microbes to resuspend in the fluid d)Oil is not used with the 100x, but rather 40x e)To prevent microbes from dryingarrow_forwarda) explain in your own words how to do a nitrate reduction test to identify unknown bacteria b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteriaarrow_forward
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