Concept explainers
To explain: The reason why white areas have formed near the rim of the red colony.
Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.
To explain: The propagation of the transcriptional state of the Ade2 gene from mother to daughter cells.
Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.
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Molecular Biology of the Cell (Sixth Edition)
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