Your lazy labmate needs your help! They set up a 10-ml culture of the slow-growing Myxococcus xanthus yesterday, but then they decided to stay up late playing videogames and won't be in until next Monday to recover. Whoops! They call you in lab that Friday and ask you to take an OD reading for them. You do this favor for them, and helpfully do the conversion into cells/mL, finding that their culture is currently sitting at about 3.0 x108 cells per mL. Your labmate is horrified: they won't be back in time before the cells hit stationary phase, since M. xanthus doubles every 5 hours. They beg you to make a new culture using these cells and set it up to reach the same cell density Monday morning. That's 59 hours away. If the cells usually lag for three hours upon dilution into fresh medium, what cell density should you dilute the culture to? About 1.3 x 107 cells/ml

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 4.2P
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Your lazy labmate needs your help! They set up a 10-ml culture of the slow-growing Myxococcus
xanthus yesterday, but then they decided to stay up late playing videogames and won't be in until
next Monday to recover. Whoops! They call you in lab that Friday and ask you to take an OD
reading for them. You do this favor for them, and helpfully do the conversion into cells/mL,
finding that their culture is currently sitting at about 3.0 x108 cells per mL. Your labmate is
horrified: they won't be back in time before the cells hit stationary phase, since M. xanthus
doubles every 5 hours. They beg you to make a new culture using these cells and set it up to
reach the same cell density Monday morning. That's 59 hours away. If the cells usually lag for
three hours upon dilution into fresh medium, what cell density should you dilute the culture to?
About 1.3 x 107 cells/ml
About 1.3 x 105 cells/ml
About 1.3 x 106 cells/ml
About 1.3 x 104 cells/ml
Transcribed Image Text:Your lazy labmate needs your help! They set up a 10-ml culture of the slow-growing Myxococcus xanthus yesterday, but then they decided to stay up late playing videogames and won't be in until next Monday to recover. Whoops! They call you in lab that Friday and ask you to take an OD reading for them. You do this favor for them, and helpfully do the conversion into cells/mL, finding that their culture is currently sitting at about 3.0 x108 cells per mL. Your labmate is horrified: they won't be back in time before the cells hit stationary phase, since M. xanthus doubles every 5 hours. They beg you to make a new culture using these cells and set it up to reach the same cell density Monday morning. That's 59 hours away. If the cells usually lag for three hours upon dilution into fresh medium, what cell density should you dilute the culture to? About 1.3 x 107 cells/ml About 1.3 x 105 cells/ml About 1.3 x 106 cells/ml About 1.3 x 104 cells/ml
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