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Average plaques for bacteriophage A，B，C are 137，36，25. PFU/ml is average plaques multiply by the volume dilution multiply by the dilution factor. Show your working for each bacteriophage.

Average plaques for bacteriophage A，B，C are 137，36，25. PFU/ml is average plaques multiply by the volume dilution multiply by the dilution factor. Show your working for each bacteriophage.

Basic Clinical Laboratory Techniques 6E
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter3: Basic Hemostasis
Section3.2: Disorders Of Hemostasis
Problem 11RQ
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Average plaques for bacteriophage A，B，C are 137，36，25. PFU/ml is average plaques multiply by the volume dilution multiply by the dilution factor. Show your working for each bacteriophage.
Experimental set up: all culture plates were incubated at 37°C over night Bacteriophage A 200 μl of bacteriophage A was added to 800 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-4. 100 µl of the mixed culture was then added to 9.9 ml molten agar and poured into the petri dish. Bacteriophage B 100 μl of bacteriophage B was added to 900 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-5. 1000 ul of the mixed culture was then added to 9 ml molten agar and poured into the petri dish. Bacteriophage C 250 μl of bacteriophage C was added to 750 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-6. 1000 μl of the mixed culture was then added to 9 ml molten agar and poured into the petri dish.
Transcribed Image Text:Experimental set up: all culture plates were incubated at 37°C over night Bacteriophage A 200 μl of bacteriophage A was added to 800 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-4. 100 µl of the mixed culture was then added to 9.9 ml molten agar and poured into the petri dish. Bacteriophage B 100 μl of bacteriophage B was added to 900 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-5. 1000 ul of the mixed culture was then added to 9 ml molten agar and poured into the petri dish. Bacteriophage C 250 μl of bacteriophage C was added to 750 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-6. 1000 μl of the mixed culture was then added to 9 ml molten agar and poured into the petri dish.
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