You know from your calculationsthat only a small proportion of the human genome is represented, even when the entire class results areconsidered. Therefore, the chance of finding a particular single-copy gene in your library is very small.Outline a strategy for constructing a genomic DNA library more representative of the entire humangenome. You will need to consider alternative vectors and the efficiency of transformation of thebacterial cells.

You know from your calculationsthat only a small proportion of the human genome is represented, even when the entire class results areconsidered. Therefore, the chance of finding a particular single-copy gene in your library is very small.Outline a strategy for constructing a genomic DNA library more representative of the entire humangenome. You will need to consider alternative vectors and the efficiency of transformation of thebacterial cells.

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 1CT

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Arabidopsis thaliana has among the smallest genomes in higher plants, with a haploid genome size of about 100 Mb. If this genome is digested with BbvCl, a restriction enzyme which cuts at the sequence CCTCAGC GGAGTCG 1. approximately how many DNA fragments would be produced? Assume the DNA has a random sequence with equal amounts of each base.
Outline a strategy for constructing a genomic DNA library more representative of the entire humangenome. You will need to consider alternative vectors and the efficiency of transformation of thebacterial cells
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