Which of the following is NOT an advantage of the PCR method over traditional cloning methods for producing desired DNA sequences? PCR is a partialy automated technique so less prone to human error PCR uses DNA polymerase to generate new DNA sequences PCR is much faster than traditional methods of cloning DNA in order to amplify it PCR does not require the production of recombinant organisms to amplify the DNA

Which of the following is NOT an advantage of the PCR method over traditional cloning methods for producing desired DNA sequences? PCR is a partialy automated technique so less prone to human error PCR uses DNA polymerase to generate new DNA sequences PCR is much faster than traditional methods of cloning DNA in order to amplify it PCR does not require the production of recombinant organisms to amplify the DNA

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 10SQ

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Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerase
Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?
Which one of the following reagents is not required in a PCR sample that would support amplification? Agarose Forward PCR primer Reverse PCR primer Taq Polymerase Template DNA
Which of the following correctly describes a difference between Sanger sequencing and PCR? O Sanger sequencing does not involve the use of primer, while PCR does. O Sanger sequencing is in vitro DNA synthesis, while PCR is in vivo DNA synthesis. Sanger sequencing is anabolic (synthesizing) process, while PCR is a catabolic (breaking down) process. O Sanger sequencing requires DNA polymerase, while PCR requires RNA polymerase. O DNA synthesis is terminated in Sanger sequencing by the addition of ddNTPs, while each newly synthesized PCR strand is terminated at the end of the template strand.
In a reaction tube, a PCR reaction recreates the DNA replication process inside a cell. Therefore, a PCR reaction needs to have the following essential components: DNA polymerase DNA template Forward and Reverse PCR primers dNTPs, including dATP, dCTP, dTTP, and dGTP MgCl2 True O False
Which of the following is/are true regarding a PCR reaction performed to amplify a DNA plasmid? There may be more than one correct answer; select all that apply. Two primers are needed to facilitate double stranded DNA extension. Denaturation, when the primers bind to the DNA template, is performed at the highest temperature. Extension is the step when the polymerase catalyzes nucleotide incorporation during polymerization. ATP, UTP, GTP, and CTP are all required for polymerization.
Which ingredient will allow the DNA to form cloudy clumps which can be collected with tweezers salt rubbing alcohols detergent solution water What is the purpose of PCR? To copy and then make many copies of a specific region of DNA To copy the entire human genome To make just a few copies of DNA To reveal the sequence of a piece of DNA If enzyme catalase has an optimum pH of 7.0, What do you think will happen en the pH is lowered to 3.0? The rate of its enzyme action is increased. Its enzyme action is not affected at all. The rate of its enzyme action is decreased. Its enzyme action ceases or stops.
Why is it important to sequence positive clones derived from PCR cloning? Group of answer choices Errors could have been incorporated during restriction enzyme digestion and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during plasmid DNA extraction and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during PCR and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during cloning and so it is important to verify that only the expected protein is produced.
What purpose do short DNA primers serve in PCR? O They determine which sequence of DNA is copied O They synthesize new DNA O They synthesize proteins O They separate RNA from DNA MacBook Air 80 888 F7 F6
Why is DNA polymerase a required part of PCR? O It synthesizes new DNA O It synthesizes proteins O It degrades the template DNA O It separates RNA from DNA MacBook Air
Each PCR cycle has three steps: DNA sample denaturation, primer annealing, and elongation/extension of the target DNA. Each cycle can theoretically double the original amount of DNA if the efficiency is 100%. True False
DNA fingerprinting uses a process called gel electrophoresis to separate the fragments of DNA. Once the DNA fragments are sorted, the pattern of bands can be analyzed. Gel Electrophoresis Procedure The smaller DNA fragments start to move away from the wells and the larger DNA fragments remain closer to the wells. An electric current is passed through the gel. DNA fragments are treated with a dye. A restriction endonuclease is added to the DNA. Using micropipettes, the DNA samples are added to the wells. DNA fingerprint is produced. DNA fragments are produced. The order in which a DNA fingerprint is produced using gel electrophoresis is Answer, Answer, Answer, Answer, Answer, Answer, and Answer.