DNA can be electrophoresed. all cells have RNA. DNA polymerase will replicate any bacterial DNA. all cells have DNA. the DNA primer is specific to particular target DNA sequence
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- Fill in the blank spaces below with the most appropriate terms. The word bank is not provided. DNA replication in bacterium Escherichia coli begins at a site in the DNA called At the replication fork, the strand is synthesized continuously while the strand is synthesized discontinuously (in fragments). The new DNA strand, which is synthesized discontinuously, initially consists of short DNA pieces that are called A short RNA primer at the beginning of each of the DNA fragments is synthesized by an enzyme called and this RNA primer is later removed by the enzyme called using its activity. Single-strand breaks (nicks) that are left behind in this process are sealed by the enzyme called A Moving to another question w!l save this resporse Quebon 4 InSelect the CORRECT pairing. DNA polymerase I : links Okazaki fragments together DNA helicase : unwinds both strands of DNA Primase : removes RNA primer DNA ligase : synthesizes DNA primerBIO: REPLICATION OF DNA Create a 75-nitrogenous base long DNA and replicate it completely. Make sure initiation, elongation and termination is present with the corresponding enzymes playing their specific part. Make sure to make proper labels.
- Create a 75-nitrogenous base long DNA and replicate it completely. Make sure initiation, elongation and termination is present with the corresponding enzymes playing their specific part and make sure to make proper labels.Draw out the molecular structure of a double stranded DNA molecule. This molecule is 6 bp in size. Don't forget label.Which of the following are major differences between DNA and RNA polymerases? RNA polymerase, but not DNA polymerase, requires a primer to begin synthesizing the nucleic acid polymer DNA polymerase, but not RNA polymerase, requires a primer to begin synthesizing the nucleic acid polymer RNA polymerase, but not DNA polymerase, is unique to eukaryotic cells DNA polymerase, but not RNA polymerase, is unique to eukaryotic cells DNA polymerase is involved in replication; RNA polymerase is involved in transcription 00
- Select the characteristics/descriptions of DNA polymerase. Select ALL that apply requires a primer adds nucleotides to 3' end of DNA strand adds nucleotides to 5' end of DNA strand does not require primer has 3'-to-5' exonuclease activity that allows "proofreading" of DNA strand being madeCRISPER works by creating double strand breaks in DNA at targeted locations. By adding the donor DNA, the researcher is trying to force the cell to utilize which DNA repair pathway? Base excision repair Homologous recombination Nonhomologous end-joining Nucleotide excision repairE. coli DNA polymerase IlI: represents over 90% of the DNA polymerase activity in E. coli cells. requires a free 5'-hydroxyl group as a primer. is the primary polymerase in chromosomal DNA replication. acts efficiently at nicks in DNA.
- Polymerase chain reaction is— a reaction involving a chain of different types of polymerases acting successively on a DNA. a way DNA replicates in the living cell. governed by the Henderson-Hasselbalch equation. a chain reaction mediated by polymerase to amplify DNA fragments of defined sequence. DNA polymerization without a template.CHOICES: Physical Agent Base Analogs Alkylating Agent Intercalating Agent Metal ions Biological Agent Disrupt DNA structures and can cause frameshift mutation Denatures DNA and causes breakage or cleavage of the phosphodiester bonds through heat Induces the base-pairing errors that increases the ionization and production of gaps in the strand of the DNA. Producing ROS (reactive oxygen species), hindering the DNA repair pathway. Non-coding DNA sequences, jumps from one place to another place in a genome and influence the function of genes. Ethylnitrosourea Causes cross-linking and generation of nucleotide dimers. Able to hotwire our DNA make up and cause disease by expressing genes that causes abnormal production of proteins. Fe2+ Daunorubicin 2-Aminopurine Arsenic Able to pair with adenine and is able to produces mutation. Thio-TEPAThe image below shows the replication bubble of a piece of DNA in the process of replication. However, the image only shows the DNA strands being replicated. Fill in the rest of the elements of the figure, specifically: primers, Okazaki fragments, newly replicated leading strand DNA, as well as the enzymes helicase, primase, DNA polymerase III, DNA polymerase I and ligase. Also be sure to indicate the 5’ and 3’ ends of all nucleic acid polymers.