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Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is…
A: The dilution factor is a term used to describe the ratio of the final volume over the aliquot…
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A:
Q: identify the organisms in an unknown culture. You find that the culture contains two gramnegative…
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A: A chemically defined media is a medium that has all the ingredients of known quantities.…
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A: Ans: Bioreactor It refers to any device that supports the biological environment. This is used for…
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A: Cell culture is the technique in which cells isolated from multicellular organisms are grown in…
Q: What are bioreactors ? How are large volume of culture maintained and processed in them ?
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A: All the organisms given in the question are different types of fungi. Organisms belonging to the…
Q: hypothetical
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A: The growth medium, also called as Nutrient Broth, is a kind of solution which is sterilized to get a…
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A: The agarose gel electrophoresis is a technique that is used to separate DNA fragments as per their…
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A: Harada-Mori culture technique It is a method of incubating fecal material on a filter paper strip in…
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A: Answer: Introduction:Thin layer chromatography, or TLC, is an analytical method to analyse mixtures…
Q: Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation?
A: Introduction Microbial culture is a microbiological technique that involves actively multiplying…
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A: PAGE or polyacrylamide gel electrophoresis is a separation technique under the influence of…
Q: Why
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Q: suffering severely due to alkaloid production and accumulation in the medium the
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A: Plastics are organic materials. It is derived from the Greek word “plastikos”.
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A: Bioelectrochemical systems or BES are specific systems designed to convert chemical energy present…
Q: What is a pure culture and how can one be obtained
A: Cells are the structural and functional units of life.
Q: On which of the following types of media would you expect untransformed bacteria to grow on?
A: In this question, we have to answer which kind media support the growth of untransformed bacteria.
Q: Which of the following salt solutions is hypotonic to biological potato cells? 1) 0.9% NaCI O 2)…
A: Hypotonic solution: A solution that contains fewer dissolved particles (such as salt and other…
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A: The investigation of microorganisms is significantly aided by the ability to culture them, that is,…
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A: Generation time is the time taken for the doubling of the single cell. According to data given in…
Q: What is a complex medium? and What are the basic components of a culture medium?
A: Medium is defined as the solid or liquid substratum on which the cells or organ can grow for further…
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A: Ensuring adequate light reaching a detector by using water colloidal solutions in light or X-ray…
Q: What types of compounds make up the gels used in electrophoresis?
A: The types of macromolecules usually separated on Agarose gel are : Nucleic acids-mainly DNA…
Q: Which of the following is a reason to run simultaneous tests on known positive and negative controls…
A: A negative control is a control group in an experiment that uses a treatment that will not produce…
Q: You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is…
A: The dilution factor may be expressed as the ratio of the concentration of stock solution to the…
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A: Cysteine is a nonessential amino acid. It is one of the amino acid that contains sulfur which allows…
Q: You dilute 1 mL of bacterial culture with 99 mL of water. What is the resulting dilution factor?
A: Dilution factor:In order to calculate the Dilution factor, the final volume is to be divided by the…
Q: Growth medium: You are running different experiments on microbes and need to make sure you choose…
A: A growth medium is a solid/ liquid media designed to support the growth of a population of…
Q: Why are plated media stored in an inverted position?
A: Plated media enables the isolation of microorganisms from samples or specimens.
Q: What are the advantages and disadvantages of liquid media?
A: Microbiological culture is a method of multiplying microorganisms in lab conditions on growth media.
Q: What is the principle of freezing of cell lines and primary cells. Describe the general protocol for…
A: Cryopreservation It is defined as a process of preserving cells, tissues, organs, or any type of…
Q: Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you…
A: Culture consists of appropriate substances which provides the growth to the desired organisms.
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- Which of the following is probably the cause of most human diseases on the skin which is salty? an acidophilic and halophilic bacterium an alkalophilic and halophilic bacterium a psychrophilic and thermophilic bacterium a mesophilic and halophilic bacterium a thermophilic and acidophilic bacteriumA microbiologist is working with a pure strain of bacteria isolated from a mixed sample. Which of the following would indicate that the bacterium is NOT Helicobacter pylori? Cells have flagella when viewed by flagellar staining. Secretes acids produced from metabolism of sucrose. Cells are curved rods. Produces and secretes urease enzyme. Cells stain Gram negative.Describe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. (Don't have to write the incubation period) ~ Only describe how would it look like on the Agar: Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient Agar
- A wound is swabbed and the swab is placed at 4°C after collection to stop bacterial growth until processing by the lab. Which of the following apply? The bacteria aer intracellular pathogens The bacteria are mesophiles The bacteria are acidophiles The bacteria are neutrophiles The bacteria are resistant to antibioticsA microbiologist identifies a bacterium that is capable of degrading cellulose, lignin, chitin, latex, aromatic chemicals, and keratin. It produces metabolic byproducts that give soil a musty smell as well as some that can be used as antibiotics. Which of the following genera is being described? View Available Hint(s) for Part A Pseudomonas Campylobacter Neisseria StreptomycesDescribe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutient Agar
- With your tests you now figured out that your patient has a Staphylococcus infection, and you would like to know if the infection is caused by Staphylococcus aureus or by Staphylococcus epidermis. What test can you do next to see if your patient indeed has a Staphylococcus aureus infection? O You can isolate the pathogen from the patient and grow it on a mannitol salt agar. Staphylococcus aureus can ferment mannitol turning the agar plate yellow. Staphylococcus erpidermis cannot ferment mannitol and the agar plate stays red. O You can isolate the pathogen from the patient and grow it on a mannitol salt agar. Staphylococcus epidermis can ferment mannitol turning the agar plate yellow. Staphylococcus aureus cannot ferment mannitol and the agar plate stays red. O There is no way you can distinguish between Staphylococcus aureus and Staphylococcus erpidermis O you do a Gram stain. Staphylococcus aureus will stay purple as it is Gram positive and Staphylococcus erpidermis will show up as…A lab technician is working with a bacterium in pure culture (in 5 ml of liquid media in a test tube). The bacterium is a mesophile that can infect humans. Which of the following is NOT true (with regards to temperature conditions for this bacterial culture)? Lowering the temperature to -10 deg C for at least an hour will likely kill all the bacteria. Placing the tube at 37 deg C will likely facilitate rapid growth of the bacteria. Raising the temperature to 90 deg C for at least an hour will likely kill all the bacteria. Placing the tube at 4 deg C will likely slow or halt growth of the bacteria.Which of the following media selects for gram negative bacteria by inhibiting the growth of gram positives that typically incorporate its dye component into their relatively bigger cell walls? blood agar EMB Mannitol salt agar Simmon citrate agar urease broth
- Which one of the following microorganisms would you use as a quality control check to ensure that your sterile technique method is properly working? S. aureus, a gram-positive bacterium E. coli, a gram-negative bacterium B. cereus, an endospore-forming bacterium M. leprae, an acid-fast bacterium T. pallidum, a flagellated, disease causing bacteriumWhich of these microbial structures is the most difficult to kill when attempting to sterilize instruments? bacterial spores Mycobacterium pneumoniae protozoal cysts fungal sporesWhich of the following treatments would be most useful to remove microbes from air pumped into a surgical environment? Giant magnets Filtration Gaseous glutaraldehyde Gaseous alcohol