In theory, if a 1-liter culture of rich medium is inoculated with 3 bacterial cells per milliliter, how many cells will be in the culture after 72 hours, given the generation time is 12 hours?
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- 88 Exercise 7: Selective and Differential Growth Medium and Tests Questions 1. Draw your expected results if you were to determine that your bacteria is an obligate anaerobe, an obligate aerobe, a facultative anaerobe, an aerotolerant anaerobe or a microaerophile. 2. Where is the oxidized zone in the tube of FTG? 3. Why is one zone pink in the FTG while the other zone is straw colored? 4. What purpose does resazurin play in FTG? eQuestion: Determine the amount of dehydrated medium needed to prepare 15 butts, 20 slants, and 25 butt-slants. Include amount for 2 additional units of each as excess to compensate for compounding losses.Activity 13 Urine Culture Inoculating Urine with a calibrated loop The number of microorganisms per milliliter recovered on urine culture can aid in the differential diagnosis of urinary tract infection (UTI). Plastic or wire inoculating loop available commercially, have been calibrated to deliver a known volume of liquid when handled correctly, thus enabling the microbiologist to estimate numbers of organism in the original specimen based on colony forming unit (CFU) of growth on cultures. PROCEDURE: 1st day 1. Gently swirl the specimen bottle to mix the urine specimen. 2. Label all plated media with name of patients, clinical specimen used and date. Label at the bottom of plates and not on the cover. 3. Obtain a disposable calibrated loop. 4. Dip the loop straight into the urine specimen so that the loop part is completely covered. Withdraw straight out. See fig. 16-18 5. Inoculate blood agar plate (BAP) as shown in fig.16-19 6. Incubate at 35 – 37˚C for 18 – 24 hours. 7. Dispose…
- 23:26 A ll 51%A Your answer 2- Describe briefly the factors to be considered before accepting raw materials of the food product from producer. Quality aspects, problems during sampling, safety, storage conditions, package, etc... Your answer 3- List in proper order the processing steps to produce the food product. Instead of drawing the flowchart of unit operations, write the processing steps in proper order from raw materials to final product Your answer 4- Name the heat transfer technique (s)/ machine(s) involved in processing of this food product. List its/their advantages and disadvantages. Your answerQuestion 4: Starting with a culture that contains 5.0 x 107 CFU/ml, describe a serial dilution scheme that would give a plate with 50 colonies.Question: Bacterium is gram positive, bacillus, single, short chain. How to determine the bacterium step by step through biochemical tests? Bacteria pool: B. cereus, B. subtilis, B. megaterium, C. perfringens, M. phlei, L. acidophilus, C. butyricum, C.sporogenes, L. lactis
- 10 If3 ml of culture is diluted by adding 9 ml of water and the absorbancy reading of the diluted culture is 0.082, what is the actual absorbancy?10 If 3 ml of culture is diluted by adding 9 ml of water and the absorbancy reading of the diluted culture is 0.082, what is the actual absorbancy? 11 If 0.1 ml of a 10-3 dilution of a culture is plated out and 65 colonies appear after incubation, how many CFU/ml were in the undiluted culture?Description 1. The fractions obtained from differential centrifugation are enriched but not pure. Explain how a greater degree of purification can be achieved using Density-gradient centrifugation and velocity centrifugation. 2. Explain equilibrium centrifugation.
- QUESTION: Microbes from a blood culture inoculated with blood drawn from a catheter were determined by the appearance of growth on Mannitol Salts Agar and through other tests to be Staphylococcus epidermidis. It can be concluded that: a. a bloodstream infection caused by S. epidermidis is occurring in the patient from whom the blood was drawn b. The bacteria fermented mannitol, changing the color of the growth medium from pink to yellow. c S. epidermidis is present in the sample as a normal flora contaminant d. That in order to determine if a bloodstream infection is present, these results must be compared to a blood culture of a sample drawn from a peripheral veinREMOVAL OF WATER FROM MICROBIAL CELLS USING FREEZE DRYING METHOD. Before starting the freeze-drying process, the weight of the test tube, Wi which is 15.33g and test tube with microbial cell pellets, Ww which is 34.94g were weighed using an electronic balance. Plus, final weight of the beaker with test tube and cells is 70.35g. Then, the average amount of water removal and average cell yield were calculated by using the formulas given which were 9.81g and 0.5001. As a conclusion, there was 9.81g of water had been removed by using the technique freeze-drying for 24 hours by using a freeze dryer. Question: 1.From this statement, please explain what process actually has happened during this freeze-drying method. 2.Make a clear conclusion that can made from this experiment.(explain briefly)281 Customized Subject Test 50. During an experiment, equal aliquots of Escherichia coli and Staphylococcus aureus are separately sonicated at the same level to mechanically disrupt bacterial cells. E. coli cells are disrupted, but there is minimal disruption of S. aureus cells. Which of the following bacterial structures best explains this difference? A) Cytoplasmic membrane B) Outer membrane C) Peptidoglycan layer OD) Phospholipid bilayer OE) Polysaccharide capsule