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Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…
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What is the effect of trypsin EDTA solution on monolayer cell culture preparation?
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- Why is PBS buffer preferred when preparing a monolayer cell culture?What is the basic principle of GPC (gel permeation chromatography)?Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25 flask. After trypsinization, she prepared her cells by mixing 50 µL of the cell suspension with 50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below: 1 (1) (ii) 3 (iii) 2 (iv) 4 Note: Count cells on the four labelled squares. Include cells touching the line on top and left. 18P 06. ¿66 Ⓡ Determine the number of viable and dead cells from her observation. What is the percentage of viability of this culture? Show your calculations in detail. Calculate the concentration of viable cells per mL in the original culture. Show your calculations in detail. Based on your understanding of cell culture, do you think she should proceed with subculture? Justify your answer.
- What is the principle of freezing of cell lines and primary cells. Describe the general protocol for freezing cultured cells.What is the principle of freezing of cell lines and primary cells.Describe the general protocol for freezing cultured cells.In detail.When is gelatin used in culture media preparation?
- What is the result of this biochemical test in the gelatin deep?cWhich enzyme is this testing for? What tool is used to inoculate this medium?in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following:*Main type of centrifuge *Subtype centrifuge * Speed range *Operational temperature range*Type of CentrifugationWhy might clinical medicine have an interest in understanding bacterial cell division at the molecular level? Explain why a hyperthermophile would probably not be a human pathogen. Describe four factors that may have an influence on the effectiveness of an antimicrobial treatment. Explain why 70% or 80% alcohol is more effective than 100% alcohol in controlling microorganisms.
- One of the early results shows that the post-centrifugation pellet of encapsulated cells also contains EA1 and/or Sap. Why is this not proof that Bacillus anthracis cells have both an S-layer and a capsule simultaneously? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/Can gelatin hydrolysis be correlated with the pathogenicity of a bacterium? Explain your answer. In addition to H2S production and motility, for what other test can SIM medium be used?What is the purpose of the negative and positive control in the Disc diffusion assay? Why is it important to use positive and negative controls in microbial experimentation? What does it tell us if the positive control leaves a zone of inhibition but the experimental treatment (penicillin disc) does not?