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Using aseptic techniques, list the general procedure of inoculating a broth from a plate culture.
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- List five essential procedures of the aseptic technique when transferring a broth culture to another broth.In aseptic technique, what will happen if the inoculated solid plated media is not inverted prior to incubation?Identify culture methods and conditions used for blood, wound,urine, fecal, and genital specimens. Of what importance areselective and differential growth media in pathogen detection,and what special conditions must be maintained for theisolation of anaerobic pathogens?
- What is the purpose of flame sterilizing the inoculating loop or needle before and after usingit? What is meant by ‘streak dilution’ technique? What is the purpose of placing the inoculated agar plate in an inverted position in theincubator?Describe the process of qualitative water test for coliform contamination. If shown EMB plates, be able to determine if coliforms are present or not.Identify culture methods and conditions used for blood, wound, urine, fecal, and genital specimens. Describe the standard procedure for obtaining and culturing a throat culture and a blood sample. What special precautions must be taken while obtaining the blood sample? Why is it important to process clinical specimens as rapidly as possible? What special procedures and precautions are necessary for the isolation and culture of anaerobes? Explain why direct gram stains are not typically performed on the throat, nasopharyngeal, or stool specimens.
- Using saline tubes containing 9 ml of saline (for dilutions), draw a diagram that would yield between 30 and 300 colonies from an original broth culture that contains 5 * 10^8 cells/ml. Indicate volumes used and dilutions made for each tube and plate.List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.
- Define the following terms; Sterilization, and Aseptic Technique. What are the differences between the two concepts?After a series of experiments, bacterial cultures were identified for disposal. How can these be properly decontaminated prior to disposal according to biosafety guidelines? METHOD:RATIONALE:How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial sample to/from an agar plate?