Homework Help is Here – Start Your Trial Now!
Science
Biology
Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the end of the gene (N- terminus end). (9178 9197) pRS-marker

Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the end of the gene (N- terminus end). (9178 9197) pRS-marker

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
bGH poly(A) signal (6977 6993) M13 Reverse PCDNA3.1- 9202 bp (6939) BstZ171 (6898 . 6917) EBV-rev (6887) Bsml (6844 6863) SV40PA-R (6649) BstBI (6503 6522) Neo-F (6483) Rsril SV40 ori (6364) BSSHII SV40 promoter PpuMI (3289) (5893 5912) Neo-R fl ori (5780) Smal (5778) TspMH - Xmal (5757) Avrll (5756) Stul 14000 15000 (5685. 5704) SV40pro-F BbvCI (3798) (5438 5458) PBABE 3 (5298 5319) Flori-F (5088 . 5107) Flori-R (4725 4742) BGH-rev (4709) Pmel (4704) Apal (4700) PSPOMI PaeR71 PspXI Xhol (4688) Xbal (4694) 13000 M13 rev d bej | signal R/KanR 6000
Transcribed Image Text:bGH poly(A) signal (6977 6993) M13 Reverse PCDNA3.1- 9202 bp (6939) BstZ171 (6898 . 6917) EBV-rev (6887) Bsml (6844 6863) SV40PA-R (6649) BstBI (6503 6522) Neo-F (6483) Rsril SV40 ori (6364) BSSHII SV40 promoter PpuMI (3289) (5893 5912) Neo-R fl ori (5780) Smal (5778) TspMH - Xmal (5757) Avrll (5756) Stul 14000 15000 (5685. 5704) SV40pro-F BbvCI (3798) (5438 5458) PBABE 3 (5298 5319) Flori-F (5088 . 5107) Flori-R (4725 4742) BGH-rev (4709) Pmel (4704) Apal (4700) PSPOMI PaeR71 PspXI Xhol (4688) Xbal (4694) 13000 M13 rev d bej | signal R/KanR 6000
QUESTION 2 The SARS-COV-2 pandemic resulted in the desire to design ELISA assays to either detect antibodies against SARS-CoV-2 protein (previous infection), or detect viral protein (current infection). You plan to express the mutant spike protein variant using the plasmid pictured below as your template. Here's the sequence you want to put in its place: https://www.ncbi.nlm.nih.gov/nuccore/AY429073.1?report=fasta, this is the DNA sequence you're going to buy as a gene block! But you need to add a couple things to it first so it'll get into the plasmid. To do so, you're going to need to remove the current insert (represented by the yellow arrow beginning at position 833-4867). Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the end of the gene (N- terminus end). (9178 .. 9197) pRS-marker Nrul (140) (9132) SgrDI (9015) Sspl Mlul (160) Spel (181) Ndel (416) SnaBI (522) (8761 .. 8780) Amp-R (8581) Pvul CMV-F (701 .. 721) Eco53kl (748) Sacl (750) 9000 T7 (795.. 814) Nhel (827) Bmtl (831) BsmBI (873) CMV en... 1000 CMV promoter T7 promoter 747R1RD322oriF 10008
Transcribed Image Text:QUESTION 2 The SARS-COV-2 pandemic resulted in the desire to design ELISA assays to either detect antibodies against SARS-CoV-2 protein (previous infection), or detect viral protein (current infection). You plan to express the mutant spike protein variant using the plasmid pictured below as your template. Here's the sequence you want to put in its place: https://www.ncbi.nlm.nih.gov/nuccore/AY429073.1?report=fasta, this is the DNA sequence you're going to buy as a gene block! But you need to add a couple things to it first so it'll get into the plasmid. To do so, you're going to need to remove the current insert (represented by the yellow arrow beginning at position 833-4867). Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the end of the gene (N- terminus end). (9178 .. 9197) pRS-marker Nrul (140) (9132) SgrDI (9015) Sspl Mlul (160) Spel (181) Ndel (416) SnaBI (522) (8761 .. 8780) Amp-R (8581) Pvul CMV-F (701 .. 721) Eco53kl (748) Sacl (750) 9000 T7 (795.. 814) Nhel (827) Bmtl (831) BsmBI (873) CMV en... 1000 CMV promoter T7 promoter 747R1RD322oriF 10008
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps with 1 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Genome annotation
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS