Many vaccines are heat sensitive due to their high concentration of proteins. Which of the following methods is appropriate to use to sterilize vaccines? O filtration incineration pasteurization autoclave
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- LISTEN Many vaccines are heat sensitive due to their high concentration of proteins. Which of the following methods is appropriate to use to sterilize vaccines? autoclave filtration pasteurization incinerationThe direct ELISA test requires only one antibody only one antigen both primary and secondary antibody only secondary antibody conjugated with enzyme systemThe following are the references for the indicated tests EXCEPT Select one: CLSI / Evaluation of chemotherapeutic antimicrobials Ridel and Walker / Evaluation of solid disinfectants Contaminated stainless steel wires with Hamster-adapted scrapie/ Prions decontamination Viral infectivity / PCR
- An ELISA test is useful for detecting the presence of antigens but not antibodies in a sample. true or falseFigure 1. Cytotoxicity test on S96 well plates that was monitored by ELISA reader 3- Discussion 1- Explain another methods by which cytotoxicity is measured ?Alchohol based hand sanitizers are effective against influenza. True/False?
- Why is Muller Hinton Agar (MHA) ideal for antibiotic susceptibility testingCells were incubated with chloroquine for 2 hours before stimulation with lipopolysaccharide (LPS, lg/ml). Cell viability was measured by staining cells with 3-(4,5-dimethylthiazol-2- yl)-2,5- diphenyltetrazolium bromide. TNF in the culture supernatant and the cell lysate were measured by sandwich ELISA, using specific monoclonal antibodies according to the manufacturer's instructions. To determine the level of cell-associated cytokines, cells were washed with phosphate-buffered saline (PBS) and lysed in a buffer containing 0.5% NP-40. Standard recombinant proteins were also diluted in PBS containing the same concentrations of NP-40 as the cell lysate. Cytokine synthesized were proteolytically cleaved and released into medium. The amount of cytokines in the culture supernatant and the cell lysate were presented. Results for TNF production are shown in Figure 1 (Jang et al, 2006). (Cells : PBMC: Human peripheral blood mononuclear cells, monocytes, monocytic lymphoma U-937 and leukemia…List the following reagents in the order in which they are added to an ELISA: secondary antibody, bodily fluid sample, enzyme substrate, primary antibody O Scondary antibody, bodily fluid sample, primary antibody, enzyme substrate O Enzyme substrate, primary antibody, secondary antibody, bodily fluid sample Bodily fluid sample, enzyme substrate, secondary antibody, primary antibody Bodily fluid sample, primary antibody, secondary antibody, enzyme substrate
- Give five (5) other examples of samples that are best prepared using Smear Preparation Technique (example: Human RBCs). Explain why.Briefly discuss the potential impact on the antibiotic susceptibility testing results when different agar thicknesses are used across different laboratories. What would you suggest to ensure that results between testing laboratories are comparable.A patient is O positive and his serum is agglutinating all screening and panel reagent cells at room temperature. His autocontrol is positive at room temperature also. The patient may have an antibody to a high incidence antigen. One possibility is that he may have a clinically insignificant autoanti-I. How could you prove that the patient has an anti-I antibody?