In conducting feeding trials to evaluate the effect of feed additives: a. Why is it important to include a negative control in the treatments? b. Why do u need to ensure that the number of replicates or sub-samples are sufficient?
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In conducting feeding trials to evaluate the effect of feed additives:
a. Why is it important to include a negative control in the treatments?
b. Why do u need to ensure that the number of replicates or sub-samples are sufficient?
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- describe the blue-white colony selection method in the recombinant colony screening, and present a brief argument for its use in biotechnology labs.What are the different categories of cell viability assays? Describe one of them briefly. Define the role (aim of use) of Trypan blue dye in cell culture studies.In an experiment assay, sometimes there are several dilutions involved.0.5 mL of (Bovine serum albumin) BSA is pipetted into 0.5 mL of PhosphateBuffer Solution (PBS).Then 0.2 mL of the mixture is pipetted into 0.8 mL of PBS.What is the total dilution factor for this assay?
- What is the benefit of doing a modified Furter-Meyer Test? What is the premise of this experiment? How would you know if this test yielded a positive result?A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This solution is serially diluted three times by combining 0.1 mL of each sequential dilution with 9.9 mL of liquid medium. Then 0.1 mL of the final dilution is plated in the plaque assay and yields 17 plaques. What is the initial density of bacteriophages in the original 1 mL?You work for a company that manufactures a range of soy products. A customer has ask that you provide assurance that your processing operations inactivate at least 99% of the soybean trypsin inhibitor in your products. How would you develop an assay for soybean trypsin inhibitor and use it to test products??
- what is the difference between presumptive and confirmational analysis?You decide to use a plaque assay with a human lung epithelial cell line to compare the effectiveness of the drug. The results of the plaque assay for one strain of influenza are shown below. Based on these results, what can you conclude about the relative effectiveness of WJ379 at 1 mM compared to oseltamivir at 1 mM? Briefly explain your rationale.1.a)What is the equivalence point and how does it relate to the recommended proportion of serum to blood in the heme agglutination assay? b. what would the predicted outcome be if you used too little serum in this assay? Why? c. what would the predicted outcome be if you used too much serum in assay? Why?
- The main reason/reasons that motivated the geneticengineers to use E. coli in the production of monoclonal antibodies compared to the usual techniques is/are: O a. The quality of monoclonal antibody produced by the usual method is poor. O b. The establishment of hybridomas is not easy. O. Theusual technique produces only polyclonal antibodies. O d. The isolation of lymphocytes is difficult. O e. The isolation of spleen cells is difficult.Which of the following statements is not true? a. Some RCTs may involve "cross-over" as part of their design b. Community trials may or may not involve randomization c. The participants for a randomized controlled trial must be randomly selected from the target population d. All RCT participants randomized into the treatment group and the control group must be included in data analysis, even if some of them did not complete the studyA researcher wants to compare the pathogenicity of a mutant pathogen relative to wild type in an animal model. The mutant is marked with the constitutive expression of a foc gene that turns colonies blue on X-gal agar. The input ratio of the experiment Dilue/white colonies) was 10:1. The output ratio of the infection experiment tant to d type was 1-100 a. What is the CI? Show your work. 1. Define the Cr and describe what it measures in your answer Which of these genotypes does better during infection, the mutant or the wild-type?