The main reason/reasons that motivated the genetic engineers to use E. coli in the production of monodlonal antibodies compared to the usual techniques is/are: O a The quality of monoclonal antibody produced by the usual method is poor. Ob. The establishment of hybridomas is not easy. Oc The usual technique produces only polyclonal antibodies. Od. The isolation of lymphocytes is difficult. O e. The isolation of spleen cells is difficult.
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- Which of the following best describes the approach that wasused in the first gene therapy trial for treating SCID?a. The normal ADA gene was introduced by injecting liposomesdirectly into the patients’ bodies. b. Lymphocytes were removed from a SCID patient, the normalADA gene was transferred into the lymphocytes vialiposomes,and then the lymphocytes were returned to thepatient’s body.c. Lymphocytes were removed from a SCID patient, the normalADA gene was transferred into the lymphocytes via a retrovirus,and then the lymphocytes were returned to thepatient’s body.d. None of the above describe the approach used in the trial.A plaque assay is an effective way to determine the concentration of lysogenic phage in a solution. A. True B. FalseExplain the difference in results for the RT-PCR from the CD4+ T cells and the RT-PCR from the CD8+T cells. (i.e., propose a conclusion)
- Many ELISAs that are used to identify the presence of antibody to viruses have a 3rd control (along with negative and positive). In between the rows of wells coated with antigen, are rows of cells coated with tissue culture used to grow the virus. In other words, patient serum is added to wells that contain antigen along with wells that contain tissue culture. Why do you think this might be necessary?5) The immunoblot shows probing of samples taken from a time course after treatment with the proteasome inhibitor MG132. Recall that the proteasome is responsible for selectively degrading only those proteins that have been tagged with ubiquitin. The left indicates the antibody that is used to detect protein for each panel. P53 P27 AGR2 GAPDH MG132 (h) 0 0.5 1 2 4 8 12 24 Image from Wang et al., (2019) Oncogene 38:3458-3474. a) What effect does MG132 have on each protein? What does this tell you about each of these proteins? ● P53 P27 AGR2 GAPDH b) How do we know that the differences we observe in the proteins is not due to un- equal loading of samples into each lane of the gel?EIAs are extremely sensitive diagnostic tools. Why,then, is the immunoblot (Western blot) procedure usedto confirm screening tests that are positive for humanimmunodeficiency virus (HIV)?
- Which of the following is true? a. conjugated vaccines are always those in which there is more than one vaccine anigen b. prevnar is conjugated vaccine c. conjugation involves ttaching a polysaccharide antigen to a carbohydrate carrier d. Conjugated vaccines tend to induce a poorer immune response than polysaccharide vaccine e. Pneumovax is a conjugated vaccine thank you!!!Outline the principle behind the following techniques: 1. Immunofixation 2. Southern blotting 3. Ion exchange chromatograph 4. SDS-PAGE.In relation to immunotechnology, answer the following: A- Answer the following in relation to Hybridoma technology: → What is the cancer cell line name used in hybridoma technology? → Give an example of murine Monocolonal Antibody produced by hybridoma technology? B- Give ONE main difference between: Murine MABs and fully human MABs? and explain the reason behind developing different forms of Monoclonal antibodies (chimeric MABs, humanized MABs, and fully human MABs) C- You are in an internship in one major Hospital, You were in rotation with a clinical pharmacist in oncology section and you came over a breast cancer patient. The personal doctor was making a quick visit to the patient to make sure the patient is following the therapeutic protocol and the treatment is running smoothly. The doctor wrote in the prescription sheet “Trastuzumab” to be dispensed by the In Patient Pharmacy, when the clinical pharmacist saw this he advised the doctor politely to use the…
- Have a look at the results in this SDS-PAGE gel. A MW ladder is shown. In the "control" lane, a sample of recombinant protein "LBT3" was loaded. Lane A shows proteins that have been prepared from leukocytes from a normal, healthy individual. Lanes B, C, D, E and F show protein prepared from leukocytes from individuals with lymphoma. What is the best explanation for the result shown for patient D? 200 150 100 75 50 37 20 Weight, kDa MW ladder Control AWhich statement is right the polyclonal antibody is expensive to produce the monoclonal antibody has relatively high specificity and sensitivity in western blot analysis C. monoclonal antibody rather than polyclone antibody can be used for therapy only hybridoma technology can produce monoclonal antibodies E. polyclonal antibodies are always welcome in molecular detectionA researcher wants to compare the pathogenicity of a mutant pathogen relative to wild type in an animal model. The mutant is marked with the constitutive expression of a foc gene that turns colonies blue on X-gal agar. The input ratio of the experiment Dilue/white colonies) was 10:1. The output ratio of the infection experiment tant to d type was 1-100 a. What is the CI? Show your work. 1. Define the Cr and describe what it measures in your answer Which of these genotypes does better during infection, the mutant or the wild-type?