Define what is a minimal medium and the nature of its components.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
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Define what is a minimal medium and the nature of its components.

Three different strains of Escherichia coli, respectively A, B, and C have been inoculated in three
different media, namely I, II, and III, and the growth of each bacterial strain has been followed by
spectrophotometry (Figure 1).
Strain A
Strain B
Strain C
4.5
45
Medium I
4.
3.5
25
2.5
3
Minimal medium
+ Glucose
2.5
25
1.5
15
0.5
as
05
100
200
300
400
500
600
100
200
100
00
s00
600
100
200
300
00
Time (min)
Strain A
सि
Strain B
etm
Strain B
4.5
Medium II
3.5
25
25
3
Minimal
2.5
25
medium
1.5
+ Glucose
+ Arginine
0.5
as
as
100
200
300
400
500
600
100
300
00
00
sa0
600
100
300
400
s00
600
me
Fime (mint
Strain A
Tme
StreinB
Streine
4.s
Medium III
4
4.
3.5
3.5
25
3
Minimal medium
+ Glucose
+ Lactose
2.5
25
25
1.5
15
1.
0.5
05
05
100
200
300
400
500
600
100
200
400
10
200
S00
S00
630
Time (min)
Tine imin]
Time (mini
Figure 1. Growth curves of the three E. coli strains A, B and C in three media:I, minimal medium
containing glucose; II, minimal medium containing glucose and arginine; and III, minimal
medium containing glucose and lactose. The graphs represent the variations of optical density
related to time.
Optical Density
Optical Density
Optical Density
Optical Deraty
Catial Density
Aag jeo
Transcribed Image Text:Three different strains of Escherichia coli, respectively A, B, and C have been inoculated in three different media, namely I, II, and III, and the growth of each bacterial strain has been followed by spectrophotometry (Figure 1). Strain A Strain B Strain C 4.5 45 Medium I 4. 3.5 25 2.5 3 Minimal medium + Glucose 2.5 25 1.5 15 0.5 as 05 100 200 300 400 500 600 100 200 100 00 s00 600 100 200 300 00 Time (min) Strain A सि Strain B etm Strain B 4.5 Medium II 3.5 25 25 3 Minimal 2.5 25 medium 1.5 + Glucose + Arginine 0.5 as as 100 200 300 400 500 600 100 300 00 00 sa0 600 100 300 400 s00 600 me Fime (mint Strain A Tme StreinB Streine 4.s Medium III 4 4. 3.5 3.5 25 3 Minimal medium + Glucose + Lactose 2.5 25 25 1.5 15 1. 0.5 05 05 100 200 300 400 500 600 100 200 400 10 200 S00 S00 630 Time (min) Tine imin] Time (mini Figure 1. Growth curves of the three E. coli strains A, B and C in three media:I, minimal medium containing glucose; II, minimal medium containing glucose and arginine; and III, minimal medium containing glucose and lactose. The graphs represent the variations of optical density related to time. Optical Density Optical Density Optical Density Optical Deraty Catial Density Aag jeo
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