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Calculate the rate of photosynthesis( absorption units/min) for sample 1 and 6.
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- DCPIP (2,6-dichlorophenol-indophenol), a blue dye, can act as an electron acceptor and becomes colorless when reduced. A suspension of chloroplasts is prepared, and DCPIP is added to the suspension. The sample is split into two test tubes. One test tube is exposed to light and the other is wrapped in foil (dark control). What would you observe over time comparing the light exposed and the dark control test tubes? A. The sample exposed to light stays blue. The sample kept in the dark turns colorless. B. The sample exposed to light turns colorless. The sample wrapped in foil stays blue. C. Both samples stay blue. D. Both samples turn colorless.At your work station, you have a spectrophotometer set at 600 nm to measure DCIP absorbance and four (4) test tubes containing different solutions. One of the test tubes is labelled “0” to blank/zero the spectrophotometer. The other three (3) test tubes contain a reaction mixture from a Hill reaction experiment using isolated chloroplasts and DCIP as an electron acceptor. DCIP is present in the oxidized state and exhibits a blue color with an absorbance at 600 nm. Upon receiving the electrons, DCIP is reduced to form DCIP-H2 that is colorless. These three (3) tubes, labelled “A”, “B”, and “C” were exposed to different light conditions during the Hill reaction. The Light conditions were random, including No light, Red light, and Green light. Please note that chlorophyll preferentially absorbs the light with wavelength of 660 nm (red) when compared to that of 520 nm (green). Your mission is to determine which Tube came from which experimental light condition. Tube: Tube…If a leaf containing the sameconcentration of chlorophyll as in the solution wasexposed to the same ultraviolet light, no fluorescencewould be seen. Propose an explanation for thedifference in fluorescence emission between thesolution and the leaf.
- A student prepares a cell homogenate from spinach leaves. She performs a cell fractionation experiment to determine the centrifugal force (g-force) that will yield the highest percentage of chloroplasts in the pellet fraction. She splits the spinach homogenate into 5 equal parts and centrifuges the parts for 10 min. at 500xg, 1000xg, 1500xg, 2000xg, or 2500xg. She then determines the proportion of chloroplasts in the supernatant and pellet fractions. The dependent variable in the student's experiment is: O centrifugal force (x g) O time of centrifugation O proportion of chloroplasts in pellet O spinach lcavesLine A (entire thick black line on figure) is the absorption spectrum of the most common pigment in a newly discovered plant. What would be the predominant color of the plant ? *note the thinner blue and red lines depict other pigments, chlorophyll a and b, not being asked about in this question) 700 450 Wavelength (nm) Visible Spectrum O Yellow O White O Black O Green red AbsorbanceTo assess photosynthesis, we relied heavily on a spectrophotometer to measure the color intensity of DCPIP in our solution. We set the spectrophotometer at wavelengths in the visible spectrum. What is the approximate wavelength range (in nm) for the visible spectrum? For example, the ultraviolet spectrum wavelength range is approximately 10-400 nm
- An experiment was conducted to demonstrate that chlorophyll is essential for photosynthesis. The procedure followed is given below: • A potted variegated plant was destarched. • Variegated leaves have green parts (where the cells contain chlorophyll) and white parts (where there is no chlorophyll). • A starch test was done on a leaf. • The potted plant was exposed to bright sunlight for 4–5 hours. • After 5 hours, a leaf was removed and tested for starch. How was the potted plant destarched? Explain why all the starch has disappeared during the process Mentioned in QUESTION 1. Describe the steps in the correct sequence that were followed during the starch test. Explain ONE safety precaution that should be taken when testing for starch. Draw a labelled leaf and indicate the colour change in the green and white parts of the leaf, after the second starch test.An experiment was conducted to demonstrate that chlorophyll is essential for photosynthesis. The procedure followed is given below: • A potted variegated plant was destarched. • Variegated leaves have green parts (where the cells contain chlorophyll) and white parts (where there is no chlorophyll). • A starch test was done on a leaf. • The potted plant was exposed to bright sunlight for 4–5 hours. • After 5 hours, a leaf was removed and tested for starch. ---------- 1. Draw a labelled leaf and indicate the colour change in the green and white parts of the leaf, after the second starch test.In a photosynthetic investigation, a student measured the following pH of the stroma and the inner thylakoid compartments of spinach leaf cells at the conclusion of the experiment. Source of Fluid pH (mean) Standard Error of Mean (SEM) Stroma 8.2 0.75 Inner compartment of thylakoid 5.6 0.35 Explain the reason for the differences in pH between the stroma and the thylakoid lumen.
- An experiment was done to determine how color of light and distance affects photosynthetic activity of the pond weed. Pond weed was placed in a test tube containing a solution of sodium hydrogen carbonate, and had its end cut; light was placed 50 cm away and four colors were tested (clear, red, blue, and green). After placing the light, the pondweed was given a certain amount of time to adjust before counting the bubbles, bubbles were counted for 1, 3, and 5 minutes. Only the bubbles that came out of the cut end was counted to represent the rate of photosynthesis. Below is the tabulated results. What are the possible factors or errors that could have affected clear/white light to have lower photosynthetic activity? In theory this should have the highest rate.Use the counting method to estimate the length of an elodea cell. You will need to use your calculation for the FOV at 400x total magnification. Show work and don’t forget units! Elodea Leaf at 400x total magTo ensure that the plant was kept at a constant temperature at each light intensity in this experiment: Select one or more: a. the temperature of the room was adjusted to keep the tube of Elodea submerged in water at a constant temperature with a thermometer inside the tube b. the tube containing Elodea submerged in water was surrounded by light on all sides with a thermometer inside the tube c. the tube containing Elodea submerged in water was placed in a water bath with a thermometer in the water bath
