100 50 - 400 450 500 550 600 650 700 Wavelength (nm) In the absorption spectrum shown above, what wavelength ranges are not efficiently absorbed by this photopigment? 400-450nm 450-700nm 450-650nm 650-700nm
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- The electronic excitation method in fluorescence and absorption spectroscopy is the same. What are some of the variations between using a fluorimeter to perform an excitation scan and an absorption scan? Do you think the two spectra should be similar? Describe. More info attached like protocol if neededConsider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %PART III - Table 3: Absorption Spectrum Data for Spinach Chloroplast Extract Absorbance Wavelength 620 nm 0.147 640 nm 0.058 660 nm 680 nm 700 nm 720 nm Wavelength 380 nm 400 nm 420 nm 440 nm 460 nm 480 nm Absorbance 0.759 0.923 Questions: 1.095 1.075 0.833 10.520 Wavelength 500 nm 520 nm 540 nm 560 nm 580 nm 600 nm 0.054 0.066 0.094 123 Graph: Plot the absorbance data on the graph below. Remember TAILS. Absorbance · 163 ·19 4 •514 · 166 1012 ·003 1. Your graph should have two "peaks." At the highest of the two, what wavelength was most strongly absorbed by the chloroplast pigments? What wavelength corresponds the top of the second peak? What wavelength was least absorbed by the chloroplast pigments? 2. Based on your observations, which wavelength (color) of light might be expected to generate the lowest rate of photosynthesis? 91
- An incandescent light bulb uses 60 j per second. How many Watts is that light bulb ?Using G-25 Sephadex beads (Fractionation range 1000-5000) KD, a sample of Vitamin B12 (MW ~1500 KD) will be: Fully excluded Partially included Fully included the activity of lysozyme was determined by looking at the increase in light scattering per unit time increase in absorbance per unit time. decrease in absorbance per unit time. increase in concentration per unit time.Sulfamethoxazole is a type of sulfonamide antibiotic. Its absorbance ranges from 250-300 nm in a UV spectrophotometer, which tells that it is in the UV region. What characteristic/s of sulfamethoxazole is responsible for the absorbance in the UV region? Please answer in detail and clearly. Thank you!
- explain the results of this experiment Table 1 Absorption Spectrum of Cobalt Chloride Using a Spectrophotometer Wavelength Absorbance 400 410 420 430 440 450 460 370 480 490 500 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 0.036 0.044 0.063 0.095 0.147 0.215 0.284 0.329 0.362 0.402 0.450 0.479 0.464 0.398 0.314 0.216 0.142 0.087 0.054 0.041 0.036 0.036 0.034 0.028 0.030 0.023 0.023 0.023 0.014 0.014 0.011 Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.042 3 0.018 0.92 4 0.027 0.139 5 6 0.036 0.045 0.190 0.236 7 (Unknown) 0.151why steroids do not show luminescence on thin layer chromatographic plates?“Atomic absorption spectroscopy (AAS) and UV-Vis spectroscopy is similar in principles”. Do you agree with this statement? Explain briefly.
- Draw a UV-Vis spectrum for each protein (shown in the table below) (A, B, C, and D) from 240 – 480 nm if they were all present at 20 µM.Cyclic photophosphorylation uses two photons to generate one ATP. If the AG of ATP hydrolysis is -50 kj/mol. What is the percent efficiency of cyclic photophosphorylation? F =96.5 kj/mol*V. Use c AG = -nFAV ⒸShow Transcribed Text to solve.The muscle enzyme lactate dehydrogenase catalyzes the reaction NADH and NAD+ are the reduced and oxidized forms, respectively, of the coenzyme NAD. Solutions of NADH, but not NAD+, absorb light at 340 nm. This property is used to determine the concentration of NADH in solution by measuring spectrophotometrically the amount of light absorbed at 340 nm by the solution. Explain how these properties of NADH can be used to design a quantitative assay for lactate dehydrogenase.
