Based on the knowiedge you gained from the cloning module, which of the lanes in th firure is assoriated with a digested plasmid that contained a PCR insert"
Q: To establish a standard curve for a BSA standard curve using Bradford, the spectrophotometer…
A: Proteins are composed of twenty standard amino acids attached together via peptide bonds. These…
Q: Explain the Role of telomere in regards to cancer
A: Telomeres gradually shorten with each cell division, resulting in less protection for the…
Q: How many ATP and NADH molecules would be produced during the citric acid cycle if 3 molecules of…
A: Cells are the basic unit of life. Cells derive energy from the metabolism of biomolecules such as…
Q: Which of the following is NOT a major source of protein? A. fish B. Milk C. Egg D. Rice
A: Introduction: Proteins are organic substances and polymers of amino acids. The elements present in…
Q: Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: The picture shown depicts what type of compound binding to an enzyme? A) A competitive inhibitor B)…
A: Regulatory enzymes show increased or decreased catalytic activity in response to specific types of…
Q: What intermolecular forces hold protein subunits in a quaternary structure?
A: The protein's quaternary structure is the association of several protein chains or substituents into…
Q: We use 3.16 fold dilution why this dilution schedule is important to determine unknown DNA…
A: The dilutions are the geometric series i.e Serial dilutions are prepared using same dilution step as…
Q: SCIENTIFIC PAPER INTRODUCTION ONLY- Titrimetric analysis of amino acids.
A: Amino acids are compounds containing carbon, hydrogen, oxygen, and nitrogen. They are composed of…
Q: Which of the following functions is NOT or cannot be performed by lipids? They can bind to DNA…
A: Lipids are a macro biomolecules made of fatty acid monomers, naturally occurring organic compounds…
Q: Lipid molecules contain either saturated or unsaturated fatty acids. You may have seen different…
A: Molecular structures containing fatty acids have an alkane ring and an acidic carboxylic group…
Q: How many enyzymatic reactions are there in glycolysis pathway?
A: There are 10 enzymatic reactions in glycolysis pathway.
Q: Trypsin cleaves a polypeptide backbone at the C-terminal side of Arg or Lys residues, whereas…
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: Based on the structural properties of your protein above , how resistant (or sensitive) would your…
A: Salmonella enterica serovar typhimurium produces 2-keto-3-deoxygluconate 6-phosphate in the…
Q: Question 13 Which of the following can determine location of glysosidic linkages? O Polarimetric…
A: A glycosidic linkage or bond is a type of covalent bond that joins the carbohydrate or sugar…
Q: 1. A student, halfan hour after the dinner, containing about 150 g of carbohydrates, 20 g of fat,…
A: Fats are glycerides (mono, di and tri), sterols, phospholipids and free fatty acids. it is stored in…
Q: 1. A student, halfan hourafter the dinner, containing about 150 g of carbohydrates, 20 g of fat, and…
A: Fats are glycerides (mono, di and tri), sterols, phospholipids and free fatty acids. it is stored in…
Q: . This cell is dependent solely on glucose as an energy source a.Muscle cells b.Kidney cells…
A: 1. the cell that solely dependent on glucose source is- Answer- d. brain cells Glucose in human…
Q: The structure of the dipeptide Gly-Asn is given by The structures of the amino acids Gly and Asn are…
A: Introduction Glycine (Gly - 3 letter code ) and Asparagine (Asn) are amino acids. Amino acids are…
Q: Choose all that aplly that are TRUE for the lipid bilayer: Negative mark is given to incorrect…
A: A lipid bilayer is described as a very thin layer that is made up of two different layers of…
Q: What is the name of the predominant amadori molecule product in the binding of hemoglobin and…
A: Excess glucose can react nonenzymatically with the amino groups of intracellular and extracellular…
Q: Why should starch solution be freshly prepared
A: Starch, also known as amylum, is a polymeric carbohydrate made up of several glucose units linked…
Q: Explain the meaning behind the term "reducing sugar ".
A: Any sugar that acts as a reducing agent due to the presence of its free aldehyde or ketone…
Q: Sugar is an organic molecule. Most organic molecules are non-polar compounds making them insoluble…
A: A polar molecule is a molecule containing polar bonds having non-zero dipole moments.
Q: What is the name of the molecule when glucose is bound to hemoglobin? a. Glycohemoglobin b.…
A: Hemoglobin is an iron-containing oxygen-transport metalloprotein located in almost all vertebrate…
Q: Five sweetener samples (labelled A to E) were tested by 97 individuals for the intensity of their…
A: Carbohydrates or carbs are maconutrient consisting of Carbon, hydrogen and oxygen atoms. In nature…
Q: List possible glucogenic products of amino acid degradation
A: Aminoacids are classified into two types based on their fate of conversion to glucose or ketone…
Q: 3. Which of the following is false? a) Methyl, phosphoryl, adenyl, uridylyl and adenosine…
A: Enzymes are basically proteins that are intricately packed within with the help of intramolecular…
Q: 10. Which of the following sequences would you expect to be a part of a beta turn? O PAAG O PAGA O…
A: Beta- turns are the simplest secondary structure, connecting two helices or sheets. They are…
Q: Why is it important to study or familiarize the levels of protein structure?
A: Proteins are polymers of amino acids linked by peptide/amide bonds. Levels of protein structure are…
Q: 5. A young woman decided to lose weight and abstained from fat-containing food for several months.…
A: Glucose is the primary source of energy for the body. So, under normal conditions, the glucose…
Q: Below is a structure of a? monosaccharide oligosaccharide disaccharide polysaccharide
A: Carbohydrates are the most prevalent biomolecules on the planet. Carbohydrates are largely composed…
Q: explain the concept behind capillary electrophoresis in DNA quantification.
A: Introduction: Capillary electrophoresis is a technique that is utilized to separate complex mixtures…
Q: Is it true that there is no such thing as vitamin overdose? For the fat-soluble vitamins, list down…
A: Although dying from a vitamin overdose is exceedingly rare, there were confirmed cases of death due…
Q: Hydrophobic signals after binding to the cell-surface receptors activate downstream signal…
A: Introduction: Cells do not work independently but continuously communicate with each other by…
Q: A newly synthesized protein contains a signal peptide on the N-terminus end of its polypeptide…
A: Proteins are a linear chain of amino acid sequences attached together via peptide bonds. There are…
Q: Explain the roles of transamination, oxidative deamination and the urea cycle in amino acid…
A: Transamination and deamination are two very important processes of amino acid degradation. The…
Q: As the strands are synthesized in replication, which of the following is true?
A: The process of in which DNA molecule produce exact copy or replica of itself is known as the…
Q: explains the absorption of the GLEEVEC detailed
A: Introduction: Gleevec also known as imatinib mesylate is a kinase inhibitor that inhibits protein…
Q: How can I distinguish between orthosteric, allosteric and cryptic ligand binding sites?
A: A binding site is a specific area on a macromolecule like a protein that binds to another molecule.…
Q: Once the RNA primers are replaced the fragments in the lagging strands are sealed by DNA pol I.…
A: The flow of genetic information from DNA to RNA, RNA to protein synthesis is called central dogma.…
Q: Lipid molecules contain either saturated or unsaturated fatty acids. You may have seen different…
A: A fatty acid is a carboxylic acid with an aliphatic chain.
Q: Explain the difference between aldoses and ketoses including its configuration of D and L
A: Carbohydrates, proteins, and lipids are macronutrients that supply the body with energy and basic…
Q: How many ATP and NADH molecules would be produced during the citric acid cycle if 3 molecules of…
A: Metabolic pathways are a series of process which includes chemical reactions occurring in a cell.…
Q: Two of the bypass reactions of gluconeogenesis involve: a phosphorylation of ADP using phosphate…
A: Introduction: Gluconeogenesis is the synthesis of glucose or glycogen from a non-carbohydrate…
Q: Write a possible mRNA base sequence that would lead to the production of this pentapeptide. (There…
A: mRNA is the abbreviation for messenger RNA, a type of single-stranded RNA involved in protein…
Q: Substrates for the first reaction of fatty acid synthesis are linked to dolichol phosphate carrier…
A: fatty acid synthesis is the process of creation of fatty acids from acetyl Co-A and NADPH through…
Q: Question # 1: A shampoo bottle lists "partially hydrolyzed protein" as one of its ingredients. What…
A: Almost every hair product on the market today contains protein in some way or another. Protein…
Q: The major pathway of ammonium assimilation combines two reactions in organisms with rich nitrogen…
A: Introduction: Nitrogen is cycled between organisms and inanimate environments. The principal…
Q: Could I have help with number 3: What biological process is similar in mechanism to soap emulsifying…
A: Introduction: The emulsion is a liquid preparation, that contains a mixture of two or more…
Step by step
Solved in 2 steps
- Based on the knowledge you gained from the cloning module, which of the bands in the figure is associated with a supercoiled plasmid?Based on the knowledge you gained from the cloning module, which of the bands in the figure is associated with the PCR insert after digestion of the plasmid?Clone number in this case is number 196 as shown in the images. What is the exact length of the segment of human DNA that has been inserted into the plasmid? *report the entire length of the insert, not just the sequences matching the ends and labels of wells isn't needed for answer*
- The process of DNA Replication depicted in the Polymerase Chain Reaction technology. Below are steps/components in DNA replication in cells. Briefly indicate the purpose/function of the components in DNA replication at the second column. For the third column, watch a PCR video in youtube and fill the column under PCR as to what step/components in the PCR technology corresponds to the second column. Question: In Reverse Transcription PCR, what step is added the ordinary PCR? Provide short explanation in the last column.Based on the knowledge you gained from the cloning module, which of the bands in the figure is associated with a supercoiled plasmidGive typed full explanation you made a recombinant plasmid (it is circular) that contains the sequence 5' AAGCTT in the middle of the inserted or cloned gene. that is the only place where this sequence occurs. the entire plasmif does not have 5' GAATTC. When putting this plasmid into E. coli (transformstion) what will happen? 1. plasmid will cut into several fragments 2. nothing the plasmif with remain circular 3. plasmid will be cut once making a linear dna fragment 4. will be cut once but will becomr two linear fragments
- Describe and contrast the common steps of DNA replication in vivo and the PCR reaction in vitro? In simple terms so, that I can understand. Thank youA PCR reaction was performed to amplify the CHE2 gene. The PCR primers used were designed to amplify bp 936-4,635 of a 10,908 bp plasmid. Calculate the expected PCR product size (in bp).Alu 1 EcoRI ori pKY Bgl I Tet: Tetracycline resistance gene A. What would be the donor organism to be used in this cloning? B. How would you make sure you only get your gene of interest (EGF) from donor but nothing else? C. How do you put EGF gene into pKY plasmid? What is the best RE to use when inserting the gene into pKY plasmid? What happens if you use the enzyme Bgl I to insert the gene of interest? How does it affect your cloning result? D. How do you make sure you only grow E.coli that have the gene of interest?
- You are cloning the genome of a new DNA virus into pUC18.You plate out your transformants on ampicillin plates containing X-gal and pick one blue colony and one white colony.When you check the size of the inserts in each plasmid (blueand white), you are surprised to fi nd that the plasmid fromthe blue colony contains a very small insert of approximately60 bp, while the plasmid from the white colony does notappear to contain any insert at all. Explain these results.Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?Using the plasmid map of pBCH2.0 calculate the length of the shortest DNA fragment if this plasmid was digested with the restriction enzymes PstI and EcoRV.