QUESTION 2To establish a standard curve for a BSA standard curve using Bradford, the spectrophotometer wavelength is set on which value?O the wavelength at which the BSA-Bradford complex's absorption value is the highestO the highest wavelength possibleO the wavelength at which the BSA-Bradford complex's absorption valuethe lowestO the wavelength at which the Bradford's absorption value is the highestthe wavelength at which the Bradford's absorption value is the lowestO the lowest wavelength possible

Proteins are composed of twenty standard amino acids attached together via peptide bonds. These…

Answered: To establish a standard curve for a BSA…

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter5: Biomedical Electronics: Digital

Section: Chapter Questions

Problem 5Q

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In basic spectrophotometer, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of a light beam is transmitted through 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 cm-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 0.025 mg/mL. and compare between 1 and 2
Consider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %
The electronic excitation method in fluorescence and absorption spectroscopy is the same. What are some of the variations between using a fluorimeter to perform an excitation scan and an absorption scan? Do you think the two spectra should be similar? Describe. More info attached like protocol if needed
Question 5 What is the total magnification of a sample with an ocular lens power of 15X and using a 40X objective lens?
Show all work including equations. Patient is treated AP/PA parallel-opposed fields. The fields are set up isocentrically on a 6 MV Linac, The patient's AP/PA separation is 25cm, and the field size at midplane is 14 x 26 cm (W x L). Calculate the AP field size on the skin.
The usual dose of digoxin for rapid digitalization is a total of 1.0mg, divide into two or more portion at intervals of 6 to 8 hours. How many milliliters of digoxin elixir containing 50mcg/mL would provide this dose?
What is v/Vmax ratio when [S] = 4km? %3D
The significance of using immersion oil when using 1OOX objective lens is The significance of using immersion oll when uskng 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized. the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce i. il. iii. iv. A light microscope should have the following features contrast Resolution magnification fluorescence ii. iv.
A flat (not spherical) tissue engineered skin (thickness 3.9 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.2 E-9 m?/s, and according to experiments that you've performed, you want to make sure that the concentration of oxygen in the device doesn't fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5 E-17 mol/[cell•s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?
Procedure II Leaf Sample Type Illumination Level Oxygen Concentration Change Data (ppm) Elapsed Time (s) Oxygen Change Rate (ppm/s) Sun- Adapted 0% -15.05 40.0s -0.37625 Shade-Adapted 0% -9.03 40.0s -0.22575 Leaf Sample Type Illumination Level Carbon Dioxide Concentration Change Data (ppm) Elapsed Time (s) Carbon Dioxide Change Rate (ppm/s) Sun- Adapted 0% 15.05 40.0s 0.37625 Shade-Adapted 0% 9.03 40.0s 0.22575 Procedure III Leaf Sample Type Illumination Level Oxygen Concentration Change Data (ppm) Elapsed Time (s) Oxygen Change Rate (ppm/s) Sun- Adapted 15% 5.13 40.0s 0.12825 Shade-Adapted 15% 8.80 40.0s 0.22 Are the results from Procedures II and III consistent with research findings that there are differences in chlorophyll production and enzyme activity between shade-adapted leaves and sun-adapted leaves? Explain your reasoning. Cite your results from…
The basic component of a fluoroscopic x-ray system suitable for interventional procedures consist of a generator,x-ray tube and housing including collimator and filtration,patient table,anti-scatter grid and an image receptor.Describe the key design features and function of each of these component and briefly discuss the role of automatic brightness control
C. Spectrophotometric Measurements of the Hill Reaction 1. Place 15 mL of the 0.05-mg chlorophyll/ mL suspension in a test tube. Wrap the tube with aluminum foil and chill in an ice bucket. This is the source of unheated chloroplasts. 2. Place the remaining 10 mL of the 0.05-mg chlorophyll/mL suspension in a test tube and heat until boiling using an alcohol lamp. Heating inactivates the chloroplasts. Stand the heated mixture for 5 min. to allow the debris to settle.

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