Microbiology: An Introduction
Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 5, Problem 10MCQ

Which culture uses the most glucose?

Use the following choices to answer questions.

  1. a. E. coli growing in glucose broth at 35°C with O2 for 5 days
  2. b. E. coli growing in glucose broth at 35°C without O2 for 5 days
  3. c. both a and b
  4. d. neither a nor b
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t /g = (Log Nt – Log N0) /0.301   I introduce a loopful of Escherichia coli cells (say, 1000) into 10 mL of Nutrient Broth at 8 p.m. the night before your lab. The cells were taken from a culture plate (Nutrient Agar) held at 37°C, and inoculated into broth at the same temperature. They were held at 37°C overnight in a shaking water bath. At what time would the culture reach the Stationary Phase? Recall that doubling time under optimal conditions (these are) is 20 minutes.    A growing bacterial culture has 10,000 CFU/mL at noon and 10,000,000 CFU/mL at 6 p.m. What is the generation time under these conditions? What are your assumptions?   At midnight you inoculate 10 mL of a culture of Enterococcus with 103 cells/mL into 990 mL of the same medium, held under the same conditions as the original culture. At what time would the culture reach 107 cells/mL? Assume exponential growth over the period. Assume that g=half an hour. Note: We worked a different variant of this problem in…
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Which of the following results from TSI test is FALSE?  A. Yellow slant, yellow butt, split agar means glucose, lactose and/or sucrose fermented gas produced  B. Yellow butt, red slant means glucose  only fermented  C. Black slant, red butt means peptones were catabolized  D. Red slant, black butt means glucose fermented hydrogen sulfide gas produced  E. Red butt, red slant means all sugars fermented, no gas produced

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Microbiology: An Introduction

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