Biology: Life on Earth with Physiology (11th Edition)
Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 4.2, Problem 1CSC
Summary Introduction

To determine:

What are the features of self-cells and donor cells that cause immune suppression.

Introduction:

Immune suppression is defined as the reduction in efficiency of the immune system to fight against diseases causing microorganisms. This condition occurs due to organ transplant or by medications.

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Experiment This experiment was designed to analyze the structure of a component (C3c protein) of the complement system, which is involved in the immune response against microorganisms. Purified C3c protein (molecular mass: 145 kd) was incubated in the presence of various concentrations of the reducing agent dithiothreitol (DTT) and then subjected to electrophoresis in a sodium dodecyl sulfate (SDS)-polyacrylamide gel. SDS disrupts noncovalent bonds and polypeptides are separated by size during electrophoresis. The gel was stained with Coomassie Brilliant Blue, a protein dye. The figure shows the molecular masses of intermediates and products generated by DTT treatment. Results: Molecular mass (kd) 145- 102- 75- 43- 27- [DTT] (MM) 10.00 1 2 3 4 5 6 Samples Questions 1. How many polypeptides are present in C3c? Determine their molecular masses from the figure. What was the purpose of using DTT in this experiment? What kind of bonds hold the polypeptides 2. 3. together? 4. How is the 102…

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Biology: Life on Earth with Physiology (11th Edition)

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