Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Textbook Question
Chapter 28.6, Problem 1CR
Why are agglutination tests so widely used in clinical diagnostics? How are fluorescent antibodies used to diagnose diseases? What advantages do immunofluorescent techniques have over traditional culturing?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
what is the principle of ELISA? What is the procedure of direct and indirect ELISA and what is the purpose of each variant in clinical diagnosis?
How is RT PCR used to detect Ebola? How does the actual RT PCR procedure work?
How is ELISA used to detect Ebola? What is the method? What binds to what?
How is Immunohistochemistry (IHC) used to detect Ebola? What is the method? What tissue is used, what binds to what?
Following is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300
Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial?
Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…
Chapter 28 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 28.1 - The use of personal protective equipment (PPE) is...Ch. 28.1 - Identify and discuss the standard safety...Ch. 28.1 - Prob. 1CRCh. 28.2 - Prob. 1MQCh. 28.2 - How can the spread of HAIs be controlled?Ch. 28.2 - Prob. 1CRCh. 28.3 - What are the key points necessary for proper...Ch. 28.3 - Identify culture methods and conditions used for...Ch. 28.3 - QWhy is it important to process clinical specimens...Ch. 28.4 - Describe the disc diffusion test and the Etest for...
Ch. 28.4 - What is the value of antimicrobial drug...Ch. 28.4 - QDescribe the disc diffusion test for antibiotic...Ch. 28.5 - Explain the reasons for changes in antibody titer...Ch. 28.5 - Describe the method, time frame, and rationale for...Ch. 28.5 - What advantages do monoclonal antibodies have...Ch. 28.5 - QWhy does antibody titer rise after infection? Is...Ch. 28.6 - How is the bivalence of antibodies significant for...Ch. 28.6 - What are the advantages and disadvantages of...Ch. 28.6 - Why are agglutination tests so widely used in...Ch. 28.7 - Prob. 1MQCh. 28.7 - Compare the advantages and disadvantages of EIA,...Ch. 28.7 - Prob. 1CRCh. 28.8 - What advantage(s) does nucleic acid amplification...Ch. 28.8 - How do quantitative PCR (qPCR) and qualitative PCR...Ch. 28.8 - Distinguish between quantitative and qualitative...Ch. 28.9 - Compare and contrast live attenuated vaccines,...Ch. 28.9 - Identify the advantages of alternative...Ch. 28.9 - QList the immunizations recommended for children...Ch. 28.10 - Prob. 1MQCh. 28.10 - How does the activity of each antibiotic class...Ch. 28.10 - What are the sources of aminoglycosides,...Ch. 28.10 - Antibiotics are chemically diverse antimicrobial...Ch. 28.11 - What steps in the viral maturation process are...Ch. 28.11 - Why are there fewer clinically effective...Ch. 28.11 - Why is host toxicity a common problem with...Ch. 28.12 - Identify the basic mechanisms of antibiotic...Ch. 28.12 - What does vancomycin have in common with...Ch. 28.12 - Prob. 3MQCh. 28.12 - What practices contribute to the spread of...Ch. 28 - Define the procedures you would use to isolate and...Ch. 28 - Prob. 2AQCh. 28 - Describe three important reasons why semisynthetic...Ch. 28 - Imagine yourself as a clinical microbiologist with...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What are the advantages and disadvantages of agglutinationtests versus fluorescent antibody assays? How are the latter usedto identify specific cells in complex mixtures, such as blood?arrow_forwardThe information is on the second picture and the questions are on the first which are : 3) What is the normal ( non - allergenic) function of a algae antibody and how does it accomplish this? 4) What is the non-normal ( allergenic ) function of an lgE antibody and how does it accomplish this? 5) How does the release of histamine lead to allergic symptoms ?arrow_forwardWhat does a white colony indicate during blue-white screening? Explain how the color is formed.arrow_forward
- What are the different applications of ELISA?arrow_forwardWhat are the two types of ELISA methods and how do they work? What is a chromogen?arrow_forwardWhat is the importance of employing aseptic techniques? Give an example of a situation in the laboratory that might happen when this method is not practiced.arrow_forward
- Describe the mechanism of an Indirect ELISA. Why is ELISA so sensitive? Why is it necessary to block unoccupied binding sites in the microtiter wells? Why is it important to have a positive control?arrow_forwardWhat is the purpose of performing a streak plate? What are some causes of lawn of cells?arrow_forwardWhy is the plate count method a determination of the number of viable cells?arrow_forward
- What are the ordered steps of an ELISA protocol? A. Add primary antibody->wash-> Bind sample to a surface ->Add substrate ->Add secondary antibody-enzyme conjugate ->wash B. Bind sample to support -> Add substrate -> Add primary antibody -> wash -> Add secondary antibody-enzyme conjugate -> wash C. Bind sample to a surface -> Add primary antibody -> wash -> Add secondary antibody-enzyme conjugate -> wash -> Add substrate D. Add secondary antibody-enzyme conjugate -> wash -> Add primary antibody -> wash -> Add substrate -> Bind sample to surfacearrow_forwardin an indirect elisa procedure what enzyme is used?arrow_forwardWhat is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handlearrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Surgical Tech For Surgical Tech Pos CareHealth & NutritionISBN:9781337648868Author:AssociationPublisher:Cengage
Surgical Tech For Surgical Tech Pos Care
Health & Nutrition
ISBN:9781337648868
Author:Association
Publisher:Cengage
Haematology - Red Blood Cell Life Cycle; Author: Armando Hasudungan;https://www.youtube.com/watch?v=cATQFej6oAc;License: Standard youtube license