BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 17, Problem 2DA
Summary Introduction
To determine: The number of spots that would be observed when human cells in the G2 phase of the cell cycle are subjected to the FISH analysis using the probe recognizing telomeric DNA.
Introduction: Fluorescent in situ hybridization (FISH) is used to identify gross chromosomal rearrangements. It is a molecular cytogenic technique in which fluorescent probes are used to detect DNA sequences.
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attached image is an image of the cells you would have been looking at under the microscope Count at least 50 cells. When done, there should be at least 50 marks on the table (one mark for each cell). You may need to count more than 50 to find at least one of every phase.
Calculate the proportions, and estimate the amount of time spent in each phase and subphase. The table will lead you through how this works. Take the number of cells in a particular phase, divided by the total number of cells examined (i.e., 50—or more), then multiply by 24 (the number of hours an average onion root tip cell takes to complete the entire cycle. This should give the hours a cell spends in each phase. Again, this assumes, that the entire cell cycle for onion root cells is 24 hours. This time can vary in different organisms.
FILL-IN this table with the results of your count of 50 cells
Phase/Subphase
# of cells
Calculation (fraction of cells x total hours of the cell cycle)
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What is different about mitosis in the plant cell as compared to the animal cell? Give two differences. please explain and do not copy from google
You view a mature, living plant cell such as an epidermal cell under the light microscope. You view the cell at sufficiently-high magnification and you have also stained the cell for chromatin. The cell has a nucleus, yet you cannot see chromosomes, at least in their typical ’sausage-formed’ shape as we all know them from textbooks. Why is that?
Chapter 17 Solutions
BIOLOGY
Ch. 17.1 - Prob. 1LOCh. 17.1 - Prob. 2LOCh. 17.1 - Describe the construction and uses of recombinant...Ch. 17.2 - Relate the process of DNA replication to PCR.Ch. 17.2 - Compare and contrast PCR, RT-PCR, and quantitative...Ch. 17.3 - Prob. 1LOCh. 17.3 - Prob. 2LOCh. 17.3 - Describe the pros and cons of RNA interference and...Ch. 17.4 - Explain how the universal nature of the genetic...Ch. 17.4 - Compare and contrast knockout, knockin, and...
Ch. 17.4 - Prob. 3LOCh. 17.5 - Describe the benefits of biofuel production from...Ch. 17.5 - Prob. 2LOCh. 17.5 - Prob. 3LOCh. 17.6 - Prob. 1LOCh. 17.6 - Compare and contrast FISH and gene chip...Ch. 17.6 - Describe how immunoassays can be used to diagnose...Ch. 17.7 - Describe the benefits of creating transgenic...Ch. 17.7 - Prob. 2LOCh. 17.7 - Evaluate issues on each side of the transgenic...Ch. 17 - Prob. 1DACh. 17 - Prob. 2DACh. 17 - Prob. 1IQCh. 17 - Prob. 2IQCh. 17 - You study a gene known to be important in the...Ch. 17 - What is the basis of separation of different DNA...Ch. 17 - Prob. 3UCh. 17 - FISH analysis of a breast tumor biopsy for HER2...Ch. 17 - In terms of studying gene function, what is the...Ch. 17 - The Ti plasmid of Agrobacterium usually induces...Ch. 17 - Prob. 1ACh. 17 - Which of the following statements is accurate for...Ch. 17 - Prob. 3ACh. 17 - Many human proteins, such as hemoglobin, are only...Ch. 17 - Amyloid beta is a proteolytic product of a protein...
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- The drug taxol is extracted from the bark of yew trees. It binds tightly to microtubules and stabilizes them. When added to cells, it causes much of the free tubulin to assemble into microtubules. Taxol can be used as an anticancer drug. At the molecular level, what does the drug prevent? At the cellular level, what part of cell division does it prevent? Be specific.arrow_forward(a)arrow_forwardScientists often extract DNA from the nucleus of cells for analysis or use. This process breaks the cell membrane, spins the solution to remove the large particles through high g-force, adds alcohol to make the DNA less soluble aqueous solution, then spins the solution again to pull the DNA out of solution. Consider a cell in metaphase compared to a cell in rest (not in the cell cycle leading to cell division). What properties of a metaphase cell might let you extract more DNA compared to the resting cell? Are there any that might make the extraction more difficult?arrow_forward
- In the micrograph below, you are seeing a collection of cells that have been stained with fluorescent dyes. One of the dyes appears green, and the other dye appears blue (when viewed with a fluorescence microscope). Based on the appearance of these stained cells, which of the following cell structures must the GREEN dye be staining? Endoplasmic reticulum Cytoskeleton Flagella Mitochondria Nucleus ..arrow_forwardTELOPHASE OF ANIMAL CELL describe the appearance of DNA, spindle fibers and location of the chromosomes please describe the appearance and location, do not state the process do not copy from google please 4Xarrow_forwardTELOPHASE OF ANIMAL CELL describe the appearance of DNA, spindle fibers and location of the chromosomes please describe the appearance and location, do not state the process do not copy from google pleasearrow_forward
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Cell Division - Mitosis and Meiosis - GCSE Biology (9-1); Author: Mr Exham Biology;https://www.youtube.com/watch?v=w7vp_uRA8kw;License: Standard YouTube License, CC-BY