You want to isolate staphylococci from a clinical sample, as you suspect the patient nas an infection with Staphylococcus aureus. What media would best isolate staphylococci from the other organisms in the culture?
Q: what is the procedure and materials you need to stain Staphylococcus aureus?
A: Staphylococcus aureus is a Gram-positive, round-shaped bacterium hat belongs to the Firmicutes and…
Q: y is an organism such as Staphylococcus adapted to growth on the human skin but the streptococci are…
A: When the pH and temperature of the environment are right, a creature can thrive there,
Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…
A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…
Q: If an unknown bacteria was given that was gram-positive coccus, and the catalase test was performed,…
A: Gram-positive bacteria, such as Staphylococcus aureus, are bacteria that provide a positive response…
Q: You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of…
A: S.Aureus is the most common cause of abscesses, furuncles, and cellulitis in the skin and soft…
Q: Does the Gram stain provide information regarding a bacterium's susceptibility to antimicrobial…
A: The Gram stain is performed for the determination and classification of the bacteria. In Gram…
Q: You have isolated a Gram positive cocci from a throat culture. You have determined that it is…
A: Gram-positive bacteria have a single cell wall anchored to the cell membrane by lipoteichoic…
Q: If an acid-fast bacterium is detected in a clinical sample, thenthe organism could bea) Cryptococcus…
A: Answer- Those bacteria that resist the decolorization with acid alcohol because of the nature of…
Q: Are the large numbers of microorganisms found in the mouth cause for concern? Explain.
A: We are authorized to answer one question at a time.Since you have not mentioned which one,I am…
Q: Noah wanted to transfer Staphylococcus aureus from a broth to an agar plate. He picked up the broth…
A: The aseptic techniques are applied in the laboratory setup to reduce the risk of contamination. It…
Q: Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?
A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…
Q: Suppose you are to observe an unknown bacterial slide. In your initial observation, it appears to be…
A: Bacteria are almost everywhere, but even with a microscope, they aren’t always easy to see. It is…
Q: What is the difference between the use of the shape bacillus and the name Bacillus? Staphylococcus…
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: Can you please discuss the principles of using Motility Test Medium for the detection of bacterial…
A: Bacterial motility is usually demonstrated in a semi-solid agar medium. Motile bacteria ‘swarm' in…
Q: Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition.…
A: A growth medium is a also known as a culture medium which can be solid, liquid, and even…
Q: Supposed you are to observe an unknown bacterial slide. In your initial observation, it appears to…
A: Microorganisms are defined as organisms that cannot be viewed by unaided eyes and require a…
Q: The organism tested on Blood Agar (plate shown furthest to the left) is: a. Micrococccus luteus b.…
A: INTRODUCTION Blood agar This is a solid culture medium consisting of blood, peptones and agar. In…
Q: You are givena mix culture of S. aureus, E. coli and P. aeruginosa. Besides using the streak plate…
A: The microorganisms can be differentiated from each other based on their growth characteristics,…
Q: Identify the oxidase-negative, gram negative bacillus isolated from the blood cultures of a…
A: Gram-negative bacteria are those that do not retain the crystal violet stain after gram staining.…
Q: Explain why the media where the Staphylococcus aureus is plated has turned yellow but the media…
A: Mannitol Salt Agar (MSA) is a differential and selective medium. A member of the staphylococcus…
Q: You are provided with a mixed culture of Escherichia coli and Pseudomonas aeruginosa, draw a flow…
A: Escherichia coli and Pseudomonas aeruginosa both are gram negative bacteria that can cause severe…
Q: a specimen from an infected wound has been submitted to the clinical laboratory. explain in detail…
A: Viruses, bacteria, fungus, protozoa, and helminths are the five types of disease-causing agents…
Q: What is the importance of using a test/control organism in describing the cultural characteristics…
A: The food materials on which the organism is developed is called culture medium and the development…
Q: Explain why it is important to use only a small amount of bacteria when preparing a smear
A:
Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…
A: Preparation of culture media and inoculation are two of the very sensitive steps in any…
Q: What test can be performed to differentiate between group A and group C streptococci?
A: Bacteria are a group of prokaryotic microscopic single celled organisms. They live in diverse…
Q: How would you expect the staining properties of 24-hour culture of Bacillus subtilis or the other…
A: Bacillus subtilis is a very well bacteria that is widely used as a Gram-positive bacteria model…
Q: After running a novobiocin sensitivity test, the zone of inhibition diameter measured 14 mm for one…
A: The microbiology studies about both the diseases causing microbes and beneficiary microbes, about…
Q: Which of these microbial structures is the most difficult to kill when attempting to sterilize…
A: Answer of the question given below...
Q: You are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method…
A: Microbiological research necessitates the use of pure cultures. Spreading bacteria on the surface of…
Q: You collected a sample from a patient with a suspected STI. After Gram staining, you observed this…
A: The staining of bacteria is an important method of classifying the bacteria There could be multiple…
Q: What test can help you differentiate staphylococci from streptococci? What reagent or media would be…
A: Both are gram positive bacteria.
Q: What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic…
A: Endospore stain is a differential stain that is used to image endospores in bacteria. A few genera…
Q: If there is growth and a color change in the Mannitol Salt Plate, which microorganism is it most…
A: Mannitol Salt Agar is high in nutrition because it contains beef extract and proteose peptone, which…
Q: what would you conclude if an inoculated plate had colonies between the streak lines
A: Streaking is a method of isolating a pure strain from single species of microorganism . in this the…
Q: Before using the API® Staph System, what tests should be performed on bacteria to confirm that they…
A: API Staph (analytical profile index) is a systematized procedure used for the identification of the…
Q: Pretend you want to test whether a certain bacterium can take iron away from hemoglobin. What is the…
A: Culture media Culture media is a type of media that support the growth of bacteria and fungi and…
Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…
A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…
Q: What things can be done during sample collection of a sick patient to help eliminate the culture of…
A: Normal flora is the organisms that are present in the body for normal functioning. The normal flora…
Q: what is your initial assumption of the organism causing this disease
A: Bacteria are the prokaryotic microorganism and based on the structural cell walls, Gram stain…
Q: With your tests you now figured out that your patient has a Staphylococcus infection, and you would…
A: S. epidermidis and S. aureus come under the category of bacteria and are associated with nosocomial…
Q: Based on the journal article “Interpretation of the Tube Coagulase Test for Identification of…
A: The coagulase test works on the principle that a coagulase enzyme causes the plasma to clot through…
Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…
A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.
Q: How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long…
A:
Q: explain why you would expect to see similarities in the types of colonies on the fomite plates and…
A: Organisms that are too small to be seen by the naked eye are microorganisms. These organisms can be…
Q: A mixed sample is collected from a salty environment. Stapnylococcus What is the expected result and…
A: Mannitol salt agar (MSA) is both a selective and differential media used for the isolation…
Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…
A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…
Q: What type of reaction or lab test differentiates the presence of either staphylococcus bacteria or…
A: Staphylococcus Bacteria which grows in cluster are Gram-positive (catalase-positive). Streptococcus…
Step by step
Solved in 2 steps
- With your tests you now figured out that your patient has a Staphylococcus infection, and you would like to know if the infection is caused by Staphylococcus aureus or by Staphylococcus epidermis. What test can you do next to see if your patient indeed has a Staphylococcus aureus infection? O You can isolate the pathogen from the patient and grow it on a mannitol salt agar. Staphylococcus aureus can ferment mannitol turning the agar plate yellow. Staphylococcus erpidermis cannot ferment mannitol and the agar plate stays red. O You can isolate the pathogen from the patient and grow it on a mannitol salt agar. Staphylococcus epidermis can ferment mannitol turning the agar plate yellow. Staphylococcus aureus cannot ferment mannitol and the agar plate stays red. O There is no way you can distinguish between Staphylococcus aureus and Staphylococcus erpidermis O you do a Gram stain. Staphylococcus aureus will stay purple as it is Gram positive and Staphylococcus erpidermis will show up as…in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?Microorganism that has the urease enzyme present a O red (or pink) O yellow (or peach) color in the urea agar test tubes. green black blue
- What test can help you differentiate staphylococci from streptococci? What reagent or media would be use? short answer onlywhat is the procedure and materials you need to stain Staphylococcus aureus?After the original specimen has been plated and a reported gram-negative rod was seen on the initial gram stain, what is your initial assumption of the organism causing this disease?
- you are given a mix culture of S aureus, E. coli and P. aeruginosa. besides using the streak plate method what other method could you choose to separate the bacteria? the result you will be looking for and interprete the result.You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of methicillin-resistant Staphylococcus aureus in the general population. You have received several samples and are ready to start your isolation procedures.What is the first step you would take if you detected contamination?You are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method what other methods could you use to separate the bacteria? Please state what method(s) you would use, the result you would be looking for to help identify each bacterium and the interpretation of the result.
- A culture of Staphylococcus is diluted as follows:(1) 20mL are added to 80mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-2 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?You mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do not know if you grew staph epidermis or E. coli. You found that the organism growing on the mannitol salt agar remained red after incubation. It is most likely that the organism is E.coli. a) True b) FalseYou are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of methicillin-resistant Staphylococcus aureus in the general population. You have received several samples and are ready to start your isolation procedures ,State if you use general or selective media and which specific media you would choose. How would you determine if the culture was contaminated